大鼠Albumin ELISA试剂盒(ab108790)
Key features and details
- Sensitivity: 0.74 µg/ml
- Range: 1.563 µg/ml - 12.5 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Rat
概述
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产品名称
大鼠Albumin ELISA试剂盒
参阅全部 Albumin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 3.8% 批次间 样品 n Mean SD CV% Overall 9.9% -
样品类型
Serum, Plasma -
检测类型
Competitive -
灵敏度
= 0.74 µg/ml -
范围
1.563 µg/ml - 12.5 µg/ml -
回收率
98 %
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检测时间
2h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s Albumin Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat Albumin in plasma and serum.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Biotinylated Rat Albumin Protein (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Albumin Microplate (12 x 8 well strips) 1 unit Albumin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
组织特异性
Plasma. -
疾病相关
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
细胞定位
Secreted. - Information by UniProt
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别名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
数据库链接
- Entrez Gene: 24186 Rat
- SwissProt: P02770 Rat
- Unigene: 202968 Rat
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108790于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108790.
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Competitive ELISA - Rat albumin (ab108790)Rat albumin measured in various samples showing quantity (microgram) per mL of tested sample
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108790 被引用在 5 文献中.
- Mirabito Colafella KM et al. Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage. Clin Sci (Lond) 136:675-694 (2022). PubMed: 35441670
- Wimmer I et al. Iron accumulation in the choroid plexus, ependymal cells and CNS parenchyma in a rat strain with low-grade haemolysis of fragile macrocytic red blood cells. Brain Pathol 31:333-345 (2021). PubMed: 33220123
- Ding K et al. AAV8-vectored suprachoroidal gene transfer produces widespread ocular transgene expression. J Clin Invest 129:4901-4911 (2019). PubMed: 31408444
- Zhang Y et al. Change in the Lipid Transport Capacity of the Liver and Blood during Reproduction in Rats. Front Physiol 8:517 (2017). PubMed: 28798692
- Arellano-Buendía AS et al. Urinary excretion of neutrophil gelatinase-associated lipocalin in diabetic rats. Oxid Med Cell Longev 2014:961326 (2014). PubMed: 25243053