兔CXCL13 ELISA试剂盒(BCA-1) (ab273248)
Key features and details
- Sensitivity: 0.35 ng/ml
- Range: 0.328 ng/ml - 80 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rabbit
概述
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产品名称
兔CXCL13 ELISA试剂盒(BCA-1)
参阅全部 CXCL13 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.35 ng/ml -
范围
0.328 ng/ml - 80 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 82.44 69% - 100% Plasma 74.71 69% - 85% Cell culture media 89.88 67% - 132% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rabbit -
产品概述
Rabbit CXCL13 ELISA Kit (ab273248) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit CXCL13 in serum (Rabbit CXCL13 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Rabbit CXCL13 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Rabbit CXCL13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Rabbit CXCL13 (BCA-1) coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Rabbit CXCL13 (BCA-1) Antibody 2 vials Rabbit CXCL13 (BCA-1) Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5. -
组织特异性
Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
细胞定位
Secreted. - Information by UniProt
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别名
- ANGIE
- ANGIE2
- B cell attracting chemokine 1
see all -
数据库链接
- Entrez Gene: 100144333 Rabbit
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273248于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Rabbit CXCL13 ELISA Kit (BCA-1) standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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