AB273248

兔CXCL13 ELISA试剂盒(BCA-1)

Name: Rabbit CXCL13 ELISA试剂盒 (BCA-1) (ab273248)| Abcam中文官网
Brand: Abcam Limited
SKU: AB273248
Price: 7665 CNY
Availability: InStock

Rabbit CXCL13 ELISA Kit (BCA-1)

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Rabbit CXCL13 ELISA Kit (BCA-1) is a Sandwich ELISA for the measurement of Rabbit CXCL13 (BCA-1) in Rabbit in Biofluids, Cell Culture Media samples.

查看别名

ANGIE, ANGIE2, B lymphocyte chemoattractant, B-cell chemoattractant, B-cell-attracting chemokine 1, B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1), BCA-1, BLC, BLR1L, C-X-C motif chemokine 13, CXC chemokine BLC, CXL13_HUMAN, Chemokine (C-X-C motif) ligand 13, Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant), Chemokine, CXC motif, ligand 13, SCYB13, Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant), Small inducible cytokine subfamily B, member 13, Small-inducible cytokine B13

1 Images

Sandwich ELISA - Rabbit CXCL13 ELISA Kit (BCA-1) (AB273248)

sELISA

Supplier Data

Sandwich ELISA - Rabbit CXCL13 ELISA Kit (BCA-1) (AB273248)

Rabbit CXCL13 ELISA Kit (BCA-1) standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Rabbit

检测类型

Sandwich

灵敏度

= 0.35 ng/mL

范围

0.328 - 80 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Rabbit CXCL13 ELISA Kit (ab273248) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit CXCL13 in serum (Rabbit CXCL13 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Rabbit CXCL13 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Rabbit CXCL13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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回收率

[ { "sample": "Serum", "range": "69 - 100 %", "average": "= 82.44" }, { "sample": "Plasma", "range": "69 - 85 %", "average": "= 74.71" }, { "sample": "Cell culture media", "range": "67 - 132 %", "average": "= 89.88" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273248-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273248-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Rabbit CXCL13 (BCA-1) coated Microplate (12 x 8 wells)", "number":"AB273248-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273248-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273248-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit CXCL13 (BCA-1) Standard (Lyophilized)", "number":"AB273248-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273248-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Rabbit CXCL13 (BCA-1) Antibody", "number":"AB273248-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273248-CMP03", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CXCL13 also known as B cell-attracting chemokine 1 (BCA-1) is a chemokine with a molecular weight of approximately 11 kDa. This protein plays an important mechanical role in the immune system by guiding the movement of B cells via chemotaxis. CXCL13 expression occurs mainly in secondary lymphoid tissues such as the spleen lymph nodes and mucosa-associated lymphoid tissue. Its expression is also noted in certain non-lymphoid tissues under inflammatory conditions.

Biological function summary

In the context of immune response and lymphoid tissue organization CXCL13 attracts B cells by interacting with the chemokine receptor CXCR5. These interactions support the formation of B-cell follicles essential for efficient immune responses. CXCL13 is not known to be part of a larger protein complex but instead works through its specific receptor-ligand interactions. Its role in guiding cell migration is vital for the development of secondary lymphoid structures.

Pathways

CXCL13 is significantly involved in the B-cell receptor signaling pathway. The chemokine facilitates the homing of B cells to follicles an important step in the adaptive immune response. CXCL13 closely associates with CXCR5 its specific receptor playing a part in establishing the spatial organization of lymphoid tissues. Additionally the chemokine influences the germinal center reaction an integral part of the immune pathway for antibody maturation.

CXCL13 levels have been linked to autoimmune diseases like rheumatoid arthritis and certain types of lymphomas. In rheumatoid arthritis increased CXCL13 expression correlates with disease severity related to its role in recruiting B cells that contribute to chronic inflammation. In lymphomas particularly those involving B cells CXCL13 supports the growth and survival of malignant cells. Its association with disease states often involves changes in CXCR5 expression or function contributing to disrupted B-cell localization and abnormal immune responses.

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产品实验方案

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靶点信息

See full target information CXCL13

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websiteProtocolBooklet

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兔CXCL13 ELISA试剂盒(BCA-1)

Rabbit CXCL13 ELISA Kit (BCA-1)

Sandwich ELISA - Rabbit CXCL13 ELISA Kit (BCA-1) (AB273248)