Prostaglandin E1 ELISA试剂盒(ab133024)
Key features and details
- Sensitivity: 5.58 pg/ml
- Range: 4.88 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
Prostaglandin E1 ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Low 24 53pg/ml 4.6% Medium 24 246pg/ml 9.5% High 24 1103pg/ml 13.7% 批次间 样品 n Mean SD CV% Low 8 49pg/ml 9.3% Medium 8 214pg/ml 11% High 8 737pg/ml 6.2% -
样品类型
Cell culture supernatant, Saliva, Urine, Serum, Plasma, Tissue Culture Media -
检测类型
Competitive -
灵敏度
5.58 pg/ml -
范围
4.88 pg/ml - 5000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Saliva 107.2 % - % Urine 109.9 % - % Serum 87 % - % Plasma 107.7 % - % Tissue Culture Media 90% - 110% -
检测时间
3h 0m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human
预测可用于: a wide range of other species -
产品概述
Abcam’s Prostaglandin E1 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E1 in, plasma, serum, urine, tissue culture media and other biological fluids.
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGE1 antigen and a polyclonal sheep antibody specific to PGE1. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the Alkaline Phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGE1 captured in the plate.
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说明
Prostaglandin E1 (PGE1) is synthesized from dihomo-γ-linolenic acid (DGLA). PGE1 has been shown to have a number of biological actions, including vasodilation, proliferation of vascular smooth muscle cells, platelet aggregation and has been shown to have insulin-like actions. Its effects are induced by receptor mediated elevation of cAMP. PGE1 is the major prostaglandin in semen.
Cross Reactivity
Compound Cross Reactivity PGE1 100% PGE2 6.50% PGE3 2.22% 13,14-dihydro-PGE1 1.50% PGE0 1.45% 15-keto-PGE1 1.15% 13,14-dihydro-15-keto-PGE1 0.19% PGF1α 0.14% PGF2α 0.04% 6-keto-PGF1α <0.1% PGA2 <0.1% PGD2 <0.1% PGB1 <0.1% 13,14-dihydro-15-keto-PGF2α <0.1% 6,15-keto-13,14-dihydro-PGF1α <0.1% Thromboxane B2 <0.1% Misoprostol <0.1% 2-Arachidonoylglycerol <0.1% Anandamide <0.1% -
经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit PGE1 Alkaline Phosphatase Conjugate 1 x 5ml PGE1 Standard 1 x 500µl PGE1 Antibody 1 x 5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133024于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Competitive ELISA |
Use at an assay dependent concentration.
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说明 |
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Competitive ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
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