Porcine TIMP1 ELISA试剂盒(ab273223)
Key features and details
- Sensitivity: 18 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Pig
概述
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产品名称
Porcine TIMP1 ELISA试剂盒
参阅全部 TIMP1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
18 pg/ml -
范围
16.38 pg/ml - 4000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 131.5 107% - 141% Serum 75.84 70% - 83% Plasma 93.73 77% - 109% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human, Pig -
产品概述
Porcine TIMP1 ELISA Kit (ab273223) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine TIMP1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for porcine TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine TIMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Porcine TIMP1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine TIMP1 Antibody 2 vials Porcine TIMP1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
序列相似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻译后修饰
The activity of TIMP1 is dependent on the presence of disulfide bonds. -
细胞定位
Secreted. - Information by UniProt
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别名
- Clgi
- Collagenase inhibitor
- Collagenase inhibitor, Human
see all -
数据库链接
- Entrez Gene: 7076 Human
- Entrez Gene: 396862 Pig
- Omim: 305370 Human
- SwissProt: P01033 Human
- SwissProt: P35624 Pig
- Unigene: 522632 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273223于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Porcine TIMP1 Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine TIMP1 ELISA kit (ab273223) Standard curve.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab273223 尚未被引用在任何文献中。