Porcine TGF alpha ELISA试剂盒(ab273225)
Key features and details
- Sensitivity: 0.6 pg/ml
- Range: 0.49 pg/ml - 120 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
概述
-
产品名称
Porcine TGF alpha ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.6 pg/ml -
范围
0.49 pg/ml - 120 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 128.6 126% - 133% Plasma 122.5 88% - 142% Cell culture extracts 129.9 114% - 142% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Pig -
产品概述
Porcine TGF-alpha ELISA Kit (ab273225) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine TGF-alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Porcine TGF-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Porcine TGF-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Porcine TGF alpha coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine TGF alpha Antibody 2 vials Porcine TGF alpha Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
-
功能
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. -
组织特异性
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. -
序列相似性
Contains 1 EGF-like domain. -
细胞定位
Cell membrane and Secreted > extracellular space. - Information by UniProt
-
别名
- EGF like TGF
- EGF-like TGF
- ETGF
see all -
数据库链接
- Entrez Gene: 397484 Pig
- SwissProt: Q06922 Pig
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273225于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
Porcine TGF alpha ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab273225 尚未被引用在任何文献中。