Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Porcine Osteoprotegerin ELISA试剂盒(OPG) (ab273217)

Submit a review Submit a question
Example data

    Key features and details

    • Sensitivity: 0.12 ng/ml
    • Range: 0.123 ng/ml - 30 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Pig

    概述

    • 产品名称

      Porcine Osteoprotegerin ELISA试剂盒(OPG)
      参阅全部 Osteoprotegerin 试剂盒

    • 检测方法

      Colorimetric

    • 精确度

      批次内
      样品 n Mean SD CV%
      Overall < 10%
      批次间
      样品 n Mean SD CV%
      Overall < 12%

    • 样品类型

      Cell culture supernatant, Serum, Plasma

    • 检测类型

      Sandwich (quantitative)

    • 灵敏度

      0.12 ng/ml

    • 范围

      0.123 ng/ml - 30 ng/ml

    • 回收率

      特定样本回收率
      样品类型 平均% 范围
      Serum 85.09 81% - 89%
      Plasma 103.3 90% - 120%
      Cell culture media 89.47 77% - 104%

    • 实验步骤

      Multiple steps standard assay

    • 种属反应性

      与反应: Pig

    • 产品概述

      Porcine Osteoprotegerin ELISA Kit (OPG) (ab273217) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine Osteoprotegerin in serum, plasma and cell culture supernatants.


      This assay employs an antibody specific for porcine Osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-porcine Osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteoprotegerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • 经测试应用

      适用于: Sandwich ELISAmore details

    • 平台

      Pre-coated microplate (12 x 8 well strips)

    性能

    • 存放说明

      Store at -20°C. Please refer to protocols.
    • 组件 1 x 96 tests
      20X Wash Buffer 1 x 25ml
      350X HRP-Streptavidin Concentrate 1 x 200µl
      5X Assay Diluent 1 x 15ml
      Anti-Porcine Osteoprotegerin (OPG) coated Microplate (12 x 8 wells) 1 unit
      Biotinylated Anti-Porcine Osteoprotegerin (OPG) Antibody 2 vials
      Porcine Osteoprotegerin (OPG) Standard (Lyophilized) 2 vials
      Stop Solution 1 x 8ml
      TMB Substrate Solution 1 x 12ml

    • 研究领域

    • 功能

      Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.

    • 组织特异性

      Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.

    • 疾病相关

      Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal.

    • 序列相似性

      Contains 2 death domains.
      Contains 4 TNFR-Cys repeats.

    • 翻译后修饰

      N-glycosylated. Contains sialic acid residues.
      The N-terminus is blocked.

    • 细胞定位

      Secreted.
    • Information by UniProt

    • 别名

      • MGC29565
      • OCIF
      • OPG
      • Osteoclastogenesis inhibitory factor
      • Osteoprotegerin
      • PDB5
      • TNF receptor superfamily member 11b
      • TNFRSF 11B
      • TNFRSF11B
      • TR 1
      • TR1
      • TR11B_HUMAN
      • Tumor necrosis factor receptor superfamily member 11B
      see all

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab273217于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Sandwich ELISA
    Use at an assay dependent concentration.
    说明
    Sandwich ELISA
    Use at an assay dependent concentration.

    图片

    • Example data
      Example data

      This standard curve is for demonstration only. A standard curve must be run with each assay.

    文献 (0)

    发表研究结果有使用 ab273217？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273217 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab273217.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com