Porcine IP-10 ELISA试剂盒(ab273216)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
概述
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产品名称
Porcine IP-10 ELISA试剂盒
参阅全部 IP10 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.4 ng/ml -
范围
0.41 ng/ml - 100 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 129 116% - 143% Serum 107.3 100% - 114% Plasma 80.59 69% - 90% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Pig -
产品概述
Porcine IP-10 ELISA Kit (ab273216) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IP-10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Porcine IP-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and Porcine IP-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Porcine IP-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Porcine IP-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Anti-Porcine IP-10 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine IP-10 Antibody 2 vials Porcine IP-10 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
细胞定位
Secreted. - Information by UniProt
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别名
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
see all -
数据库链接
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273216于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Porcine IP-10 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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