Porcine IFN beta ELISA试剂盒(ab273222)

Porcine IFN beta ELISA试剂盒
参阅全部 Interferon beta 试剂盒

Colorimetric

Cell culture supernatant, Serum, Plasma

Sandwich (quantitative)

2 pg/ml

1.64 pg/ml - 400 pg/ml

特定样本回收率

样品类型	平均%	范围
Cell culture supernatant	90.69	88% - 95%
Serum	86.51	82% - 89%
Plasma	71.62	68% - 75%

Multiple steps standard assay

与反应: Pig

Porcine IFN beta ELISA Kit (ab273222) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IFN beta in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Porcine IFN-beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Porcine IFN-beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Pre-coated microplate (12 x 8 well strips)

Store at -20°C. Please refer to protocols.

• Cardiovascular
• Angiogenesis
• Cytokines
• Other

• Immunology
• Innate Immunity
• Cytokines
• Interferons

• Cancer
• Tumor immunology
• Cytokines
• Interferons

• Neuroscience
• Diseases

• Neuroscience
• Processes