Porcine FGF19 ELISA试剂盒(ab273220)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 20.48 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
概述
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产品名称
Porcine FGF19 ELISA试剂盒
参阅全部 FGF19 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
10 pg/ml -
范围
20.48 pg/ml - 5000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 112.9 98% - 145% Serum 141.8 135% - 149% Plasma 139.2 127% - 146% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Pig -
产品概述
Porcine FGF19 ELISA Kit (ab273220) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine FGF-19 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for porcine FGF-19 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF-19 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine FGF-19 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF-19 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Porcine FGF19 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine FGF19 Antibody 2 vials Porcine FGF19 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB. -
组织特异性
Expressed in fetal brain, cartilage, retina, and adult gall bladder. -
序列相似性
Belongs to the heparin-binding growth factors family. -
细胞定位
Secreted. - Information by UniProt
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别名
- FGF 19
- FGF-19
- FGF15
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273220于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Porcine FGF-19 Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine FGF-19 ELISA kit (ab273220) Standard curve.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab273220 被引用在 1 文献中.
- Cortes-Araya Y et al. KLB dysregulation mediates disrupted muscle development in intrauterine growth restriction. J Physiol 600:1771-1790 (2022). PubMed: 35081669