Porcine Decorin ELISA试剂盒(ab273221)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Pig
概述
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产品名称
Porcine Decorin ELISA试剂盒
参阅全部 Decorin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
4 pg/ml -
范围
4.1 pg/ml - 1000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 97.43 93% - 104% Serum 116.8 92% - 142% Plasma 93.49 67% - 91% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human, Pig -
产品概述
Porcine Decorin ELISA Kit 9ab273221)) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine Decorin in serum, plasma (Collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as anti-coagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for porcine Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Porcine Decorin coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine Decorin Antibody 2 vials Porcine Decorin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
May affect the rate of fibrils formation. -
疾病相关
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. -
序列相似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
翻译后修饰
The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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别名
- Bone proteoglycan II
- CSCD
- DCN
see all -
数据库链接
- Entrez Gene: 1634 Human
- Entrez Gene: 396957 Pig
- Omim: 125255 Human
- SwissProt: P07585 Human
- SwissProt: Q9XSD9 Pig
- Unigene: 728830 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273221于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Porcine Decorin Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine Decorin ELISA kit (ab273221) Standard curve.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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