Porcine CCL4 ELISA试剂盒(ab273215)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
概述
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产品名称
Porcine CCL4 ELISA试剂盒
参阅全部 CCL4/MIP-1 beta 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Plasma, Tissue -
检测类型
Sandwich (quantitative) -
灵敏度
0.2 ng/ml -
范围
0.205 ng/ml - 50 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 111.3 106% - 118% Plasma 104.8 93% - 121% Cell culture media 122.1 115% - 133% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Pig -
产品概述
Porcine CCL4 ELISA Kit (ab273215) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine CCL4 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Porcine CCL4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Porcine CCL4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Porcine CCL4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Porcine CCL4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Anti-Porcine CCL4 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine CCL4 Antibody 2 vials Porcine CCL4 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
翻译后修饰
N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
细胞定位
Secreted. - Information by UniProt
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别名
- MIP 1 beta
- Secreted protein G 26
- ACT 2
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273215于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Porcine CCL4 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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