Phosphotyrosine In-Cell ELISA试剂盒(ab126431)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Colorimetric
- Sample type: Adherent cells
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Phosphotyrosine In-Cell ELISA试剂盒 -
检测方法
Colorimetric -
样品类型
Adherent cells -
检测类型
Cell-based (qualitative) -
检测时间
5h 10m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse, Rat, Human -
产品概述
ab126431 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Phosphotyrosine and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines. By determining Phosphotyrosine protein in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.
In the Cell-Based Phosphotyrosine ELISA kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, HRP-Anti- Phosphotyrosine is pipetted into the wells and incubated. The wells are washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests HRP Conjugated Anti-Phosphotyrosine Concentrate (2000X) 1 x 7µl Blocking Buffer Concentrate (5X) 1 x 20ml Fixing Solution 1 x 30ml Quenching Buffer Concentrate (30x) 1 x 2ml Stop Solution 1 x 14ml TMB One-Step Substrate Reagent 1 x 12ml Uncoated 96-well Microplate 1 unit Wash Buffer A Concentrate (20X) 1 x 30ml Wash Buffer B Concentrate (20X) 1 x 30ml -
研究领域
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相关性
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126431于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
图片
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In-Cell ELISA - Phosphotyrosine In-Cell ELISA Kit (ab126431)
A431 cells were treated for 30 min with 50 µL of 100 mM Genistein or 2 mM Lavendustin in appropriate wells at room temperature prior to EGF stimulation. Added 50 µL different concentrations of rhEGF (0, 20 or 100 ng/ml in serum free DMEM) to appropriate wells. Then incubated for 10 min at 37°C.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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