Phospho-TYK2 (Y1054) and Total TYK2 ELISA试剂盒

产品详情

Phospho-TYK2 (Y1054) and Total TYK2 ELISA Kit (ab279963) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated TYK2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-TYK2 and total TYK2. An anti-pan TYK2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and TYK2 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho TYK2 (Y1054) antibody is added to detect phosphorylated TYK2. In the remaining wells, biotinylated anti-pan-TYK2 antibody is used to detect pan TYK2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TYK2 (Y1054) or pan TYK2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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