Phospho S232 PDH E1 alpha蛋白(PDHA1) Profiling ELISA试剂盒(ab115343)
Key features and details
- Sensitivity: 31 µg/ml
- Range: 31 µg/ml - 1000 µg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Cow, Human
概述
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产品名称
Phospho S232 PDH E1 alpha蛋白(PDHA1) Profiling ELISA试剂盒
参阅全部 PDHA1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 8 = 8.3% 批次间 样品 n Mean SD CV% 1 4 = 7.7% -
样品类型
Cell culture extracts, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
= 31 µg/ml -
范围
31 µg/ml - 1000 µg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse, Rat, Cow, Human -
产品概述
ab115343 is an in vitro enzyme-linked immunosorbent assay to determine the levels of phospho S232 PDHA1 protein in cell and tissue lysates. The assay employs a mouse antibody specific for PDHA1 protein coated on a 96-well plate. Samples are pipetted into the wells and PDHA1 protein present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a rabbit anti-phospho S232 PDHA1 protein detector antibody is added. After washing away unbound detector antibody, HRP-conjugated anti-rabbit antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of phospho S232 PDHA1 protein bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
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说明
Store all components at 4°C. The kits are stable for at least 6
months. Unused microplate strips should be returned to the pouch
containing the desiccant and resealed.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X HRP Label 1 x 1ml 10X phospho S232 PDHA1 protein Detector Antibody 1 x 0.7ml 20X Buffer 1 x 20ml Extraction Buffer (ab260490) 1 x 15ml Microplate 96 antibody coated wells in 12 strips 1 unit HRP Development Solution 1 x 12ml -
研究领域
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功能
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). -
组织特异性
Ubiquitous. -
疾病相关
Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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别名
- ODPA_HUMAN
- PDH
- PDHA
see all -
数据库链接
- Entrez Gene: 407109 Cow
- Entrez Gene: 5160 Human
- Entrez Gene: 18597 Mouse
- Entrez Gene: 29554 Rat
- Omim: 300502 Human
- SwissProt: A7MB35 Cow
- SwissProt: P08559 Human
- SwissProt: P35486 Mouse
see all
相关产品
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SimpleStep ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115343于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)
The PDHA1 bound from undosed HeLa cells was subject to in-well kinase treatment (PDK1&3) or in-well phosphatase treatment (PDP1) according to the supplementary protocol shown below. Untreated cells showed a significant endogenous phosphorylation signal at S232 which is only slightly increased by kinase treatment. Conversely phosphatase treatment was able to significantly reduce the phospho S232 signal from the endogenous levels.
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ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)HeLa cells were cultured for 4 hours in media supplemented with DCA (20mM) to specifically inhibit mitochondrial PDH kinases, or NaF (20mM), a general inhibitor of serine/threonine protein phosphatases. The DCA treatment reduced the level of phospho S232. Conversely NaF treatment, to inhibit cellular serine phosphatases, increased the phosphorylation level of S232. -
ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)Example control sample curve for HepG2 cell extract. The phosphorylation state of PDHA1 can vary by treatment but also by cell culture conditions such as media supplements, nutrients and also cell density.
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ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)Example control sample curve for HeLa cell extract. The phosphorylation state of PDHA1 can vary by treatment but also by cell culture conditions such as media supplements, nutrients and also cell density.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab115343 被引用在 4 文献中.
- Yang Z et al. Phosphorylated form of pyruvate dehydrogenase a1 mediates tumor necrosis factor a-induced glioma cell migration. Oncol Lett 21:176 (2021). PubMed: 33574915
- Shi Y et al. Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca2+]m-PDP1-PDH-histone acetylation retrograde signaling. Cell Death Dis 12:837 (2021). PubMed: 34489398
- Ahn E et al. Temporal fluxomics reveals oscillations in TCA cycle flux throughout the mammalian cell cycle. Mol Syst Biol 13:953 (2017). PubMed: 29109155
- Zhang W et al. Dicumarol inhibits PDK1 and targets multiple malignant behaviors of ovarian cancer cells. PLoS One 12:e0179672 (2017). PubMed: 28617852