Phospho-Raf1 (S301) and Total Raf1 ELISA试剂盒

产品详情

Phospho-Raf1 (S301) and Total Raf1 ELISA Kit (ab279904) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Raf1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Raf1 and total Raf1. An anti-pan Raf1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Raf1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho Raf1 (S301) antibody is added to detect phosphorylated Raf1. In the remaining wells, biotinylated anti-pan-Raf1 antibody is used to detect pan Raf1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Raf1 (S301) or pan Raf1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.