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AB279822

Phospho-Hsp27 (S82) ELISA试剂盒

Phospho-Hsp27 (S82) ELISA Kit

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Phospho-Hsp27 (S82) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-Hsp27 (S82) in Human in Cell/Tissue Extracts samples.

查看别名

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

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Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)

Positive Control.

HeLa cells were treated with PMA at 37°C for 20 mins.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)

HeLa cells treated/untreated with PMA. HeLa cells were untreated or treated with 250 nM PMA for 20 mins.

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)

HeLa cells treated/untreated with PMA. HeLa cells were untreated or treated with 250 nM PMA for 20 mins.

关键信息

检测方法

Colorimetric

样品类型

Cell Lysate

反应种属

Human

检测类型

Semi-quantitative

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Phospho-Hsp27 (S82) ELISA Kit (ab279822) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Hsp27 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Hsp27. An anti-pan Hsp27 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Hsp27 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-Hsp27 (S82) antibody is used to detect phosphorylated Hsp27. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Hsp27 (S82) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279822-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279822-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279822-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279822-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279822-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-Phospho-Hsp27 Coated Microplate", "number":"AB279822-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-Hsp27 (S82)-antibody", "number":"AB279822-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279822-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated HeLa cell lysate", "number":"AB279822-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279822-CMP08", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
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产品实验方案

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靶点信息

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).
See full target information HSPB1 phospho S82
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