Anti-Parvovirus B 19 IgG ELISA试剂盒(ab178650)
Key features and details
- Sensitivity: 95 %
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
概述
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产品名称
Anti-Parvovirus B 19 IgG ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Pos. Serum 8 6.9% 批次间 样品 n Mean SD CV% Pos. Serum 20 6.4% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Indirect -
灵敏度
> 95 % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s anti-Parvovirus B 19 IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against -Parvovirus B 19 in Human serum and plasma.
A 96-well plate has been precoated with Parvovirus B 19 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Parvovirus B 19 antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Parvovirus B 19 IgG sample captured in plate.
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经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Parvovirus B 19 (IgG) Coated Microplate (12 x 8 wells) 1 unit Parvovirus B 19 Anti-IgG HRP Conjugate 1 x 20ml Parvovirus B 19 IgG Cut-off Control 1 x 3ml Parvovirus B 19 IgG Negative Control 1 x 2ml Parvovirus B 19 IgG Positive Control 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究领域
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相关性
Parvoviruses are cubic single-stranded DNA viruses of about 18-32 nm lacking an envelope. Parvovirus B19 infects only humans, and since there are no crossreactivities between animal parvoviruses and B19, transmission between pets and humans is not possible. Parvovirus B19 is the causative agent of Erythema infectiosum, the so-called “fifth disease”, a mild rash illness that occurs most commonly in children. Infected persons are contagious during the early part of the illness before the rash appears so in adults the rate of epidemia amounts to about 60%. About 20% of adults and children who are infected with parvovirus B19 do not develop any symptoms. Persons infected with the virus, however, do develop lasting immunity that protects them against infection in the future. Parvovirus B19 infection may cause a serious illness in persons with sickle-cell disease or similar types of chronic anemia as well as in persons who have problems with their immune system (people with leukemia or cancer, who are born with immune deficiencies, who have received an organ transplant, or who have HIV infection). Occasionally (less than 5% of all pregnant women infected with parvovirus B19) serious complications may develop during pregnancy: risk of Morbus haemolyticus fetalis.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178650于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
图片
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
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SDS download
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Datasheet download
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