p70S6K (pT389 + Total) ELISA试剂盒(ab225582)
Key features and details
- One-wash 90 minute protocol
- Sample type: Cell Lysate, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Mouse, Human
概述
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产品名称
p70S6K (pT389 + Total) ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% pT389 6 3.3% Total 6 4.5% 批次间 样品 n Mean SD CV% pT389 3 2.9% Total 3 4.1% -
样品类型
Cell Lysate, Tissue Homogenate -
检测类型
Semi-quantitative -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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产品概述
Abcam’s p70S6K (pT389) and p70S6K (Total) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of p70S6K (pT389) and Total p70S6K protein in Human and mouse cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Buffer PT 1 x 15ml 50X Cell Extraction Enhancer Solution 1 x 1ml 5X Cell Extraction Buffer PTR 1 x 12ml Lyophilized p38 MAPK alpha Control Lysate 1 vial p70S6K (pT389) Capture Antibody 1 x 1.5ml p70S6K (pT389) Detector Antibody 1 x 1.5ml p70S6K (Total) Capture Antibody 1 x 1.5ml p70S6K (Total) Detector Antibody 1 x 1.5ml Plate Seal 1 unit SimpleStep Pre-Coated 96 Well Microplate (12 x 8 well strips) 1 unit Stop Solution 1 x 12ml TMB Substrate 1 x 12ml -
研究领域
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细胞定位
P70 S6 Kinase: Cell junction › synapse › synaptosome By similarity. Mitochondrion outer membrane. Mitochondrion. Note: Colocalizes with URI1 at mitochondrion. Isoform Alpha I: Nucleus. Cytoplasm Isoform Alpha II: Cytoplasm -
数据库链接
- Entrez Gene: 6198 Human
- Entrez Gene: 6199 Human
- Entrez Gene: 58988 Mouse
- Entrez Gene: 72508 Mouse
- Entrez Gene: 361696 Rat
- Entrez Gene: 83840 Rat
- Omim: 608938 Human
- Omim: 608939 Human
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225582于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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Example of p70S6K (pT389) and p70S6K (Total) cell lysate standard curve
Background-subtracted data values (mean +/- SD) are graphed.
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Example of p70S6K (pT389) recombinant protein standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Cell line analysis for Total p70S6K from 100 µg/mL preparations of cell extractsData from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
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Inhibition of p70S6K (pT389) phosphorylation in MCF-7 cells in response to wortmannin treatmentMCF-7 cells were cultured in 96-well tissue culture plates, and treated (2 h) with a dose-range of wortmannin before stimulation with insulin (10 µg/mL for 30 min) and cell lysis. Data from triplicate measurements of p70S6K (pT389) are plotted and compared against total p70S6K protein levels.
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Sandwich ELISA - p70S6K (pT389 + Total) ELISA Kit (ab225582)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - p70S6K (pT389 + Total) ELISA Kit (ab225582)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
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