Oxytocin ELISA试剂盒(ab133050)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Tissue Culture Media, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
Oxytocin ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Low 20 39.9pg/ml 12.6% Medium 20 121.4pg/ml 10.2% High 20 363.7pg/ml 13.3% 批次间 样品 n Mean SD CV% Low 17 47pg/ml 20.9% Medium 17 145.1pg/ml 16.5% High 17 397.2pg/ml 11.8% -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue Culture Media, Cerebral Spinal Fluid -
检测类型
Competitive -
灵敏度
15 pg/ml -
范围
15.6 pg/ml - 1000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Saliva 90 % - % Urine 111 % - % Serum 104 % - % -
检测时间
25h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human
预测可用于: a wide range of other species -
产品概述
Abcam’s Oxytocin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Oxytocin in Tissue Culture media, Human Serum, Saliva, Cerebrospinal Fluid and Urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Oxytocin antigen and a polyclonal rabbit antibody specific to Oxytocin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Oxytocin captured in the plate.
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说明
Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance.
Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. In the Oxytocin-like peptide, mesotocin, a common peptide found in some fishes, reptiles, amphibians, marsupials and nonmammalian tetrapods, the leucine at residue 8 is substituted for isoleucine. Acting in classical endocrine fashion, Oxytocin elicits regulatory effects by binding specific cell surface receptors which in turn initiate a secondary intracellular response cascade via a phosphoinositide signaling pathway.
Cross Reactivity
Compound % Cross Reactivity Mesotocin 7.0 Oxytocin 100 Arg8-Vasotocin 7.5 Ser4,Ile8-Oxytocin <0.2 TRH <0.2 Somatostatin <0.2 Met-Enkephalin <0.2 VIP <0.2 Lys8-Vasopressin <0.2 Arg8-Vasopressin <0.2 α−ANP <0.2 Growth Hormone <0.2 Tocinoic acid <0.2 Melanostatin <0.2 -
经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Oxytocin Alkaline Phosphatase Conjugate 1 x 5ml Oxytocin Antibody 1 x 5ml Oxytocin Standard 1 x 0.5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
研究领域
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细胞定位
Secreted -
别名
- Ocytocin
- OT
- OT NPI
see all -
数据库链接
- Entrez Gene: 5020 Human
- Omim: 167050 Human
- SwissProt: P01178 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133050于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Competitive ELISA |
Use at an assay dependent concentration.
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说明 |
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Competitive ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab133050 被引用在 10 文献中.
- Veiga L et al. Depressive symptomatology, temperament and oxytocin serum levels in a sample of healthy female university students. BMC Psychol 10:36 (2022). PubMed: 35193693
- Late-Breaking Abstracts for ECO2022. Obes Facts 15 Suppl 1:241-286 (2022). PubMed: 35500565
- 58th EASD Annual Meeting of the European Association for the Study of Diabetes : Stockholm, Sweden, 19 - 23 September 2022. Diabetologia 65:1-469 (2022). PubMed: 35920845
- Eerola T et al. Being moved by listening to unfamiliar sad music induces reward-related hormonal changes in empathic listeners. Ann N Y Acad Sci N/A:N/A (2021). PubMed: 34273130
- von Erlach T et al. Robotically handled whole-tissue culture system for the screening of oral drug formulations. Nat Biomed Eng 4:544-559 (2020). PubMed: 32341538
- Li C et al. Downregulation of microRNA-29c reduces pain after child delivery by activating the oxytocin-GABA pathway. Mol Med Rep 22:1921-1931 (2020). PubMed: 32705167
- Maroun M et al. Sex-dimorphic role of prefrontal oxytocin receptors in social-induced facilitation of extinction in juvenile rats. Transl Psychiatry 10:356 (2020). PubMed: 33077706
- Wälivaara DÅ et al. Effects of Lactobacillus reuteri-containing lozenges on healing after surgical removal of mandibular third molars: a randomised controlled trial. Benef Microbes 10:653-659 (2019). PubMed: 31157556
- Dayanithi G et al. Vasopressin and oxytocin in sensory neurones: expression, exocytotic release and regulation by lactation. Sci Rep 8:13084 (2018). PubMed: 30166555
- Bomann AC et al. The neurobiology of social deficits in female patients with borderline personality disorder: The importance of oxytocin. Personal Ment Health 11:91-100 (2017). Human . PubMed: 28397403