Ovine IL-12 p70 ELISA试剂盒(ab273255)
Key features and details
- Sensitivity: 2.5 pg/ml
- Range: 2.05 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Sheep
概述
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产品名称
Ovine IL-12 p70 ELISA试剂盒
参阅全部 IL-12 p70 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, EDTA Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
2.5 pg/ml -
范围
2.05 pg/ml - 200 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 72.35 68% - 79% Plasma 74.38 68% - 79% Cell culture media 94.78 80% - 114% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Sheep -
产品概述
Ovine IL-12 p70 ELISA Kit (ab273255) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Ovine IL-12 p70 in serum, plasma (Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended) and cell culture supernatants.
This assay employs an antibody specific for Ovine IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Ovine IL-12 p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Ovine IL-12 p70 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Ovine IL-12 p70 Antibody 2 vials Ovine IL-12 p70 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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相关性
IL-12 p70 is a disulfide bonded heterodimer of the IL-12 40 kDa and 35 kDa subunits. -
细胞定位
Secreted -
别名
- IL12A
- IL12B
- CLMF p35
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273255于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Ovine IL-12 p70 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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