JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB285284

Overall Exosome Capture and Quantification ELISA Assay试剂盒

Overall Exosome Capture and Quantification ELISA Assay Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Overall Exosome Capture and Quantification ELISA Assay Kit is a Sandwich ELISA for the measurement of Overall Exosome Capture and Quantification ELISA Assay Kit in in Biofluids samples.
3 Images
Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)
  • sELISA

Supplier Data

Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)

CD9 Titration of COLO1 (1) and plasma (2) exosomes. Example of standard curves obtained. Exosome standard preparation derived from (1) COLO1 Cell Culture Supernatant and (2) Plasma of healthy donors. This standard curve is for demonstration only. For quantification purposes, a standard curve must be obtained with every assay.

Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)
  • sELISA

Supplier Data

Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)

CD9 titration of plasma healthy donor exosome standards.

Western blot - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)
  • WB

Supplier Data

Western blot - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)

CD9 marker detection by Western Blotting on lyophilized exosomes from human plasma and the recombinant CD9 protein.

false

关键信息

样品类型

Plasma

检测类型

Sandwich

范围

0.78125 - 50 µg

检测平台

Microplate (12 x 8 well strips)

产品详情

Overall Exosome Capture and Quantification ELISA Assay Kit ab285284 (previously called ExoQuant Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric), K1201) is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes.

It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).

精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 13" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

规格

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab285284-96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285284-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Immunoplate (Transparent)", "number":"AB285284-CMP02", "productcode":"" }, { "size":"2 x 10 mL", "name":"Sample buffer", "number":"AB285284-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"Substrate chromogenic solutions", "number":"AB285284-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"25X Wash buffer", "number":"AB285284-CMP07", "productcode":"" }, { "size":"1 x 20 µL", "name":"Primary antibody", "number":"AB285284-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"HRP-Conjugate", "number":"AB285284-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Exosome Standards", "number":"AB285284-CMP01", "productcode":"" } ] } } }

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C

产品实验方案

靶点信息

websiteProtocolBooklet
en

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂,为您科研的每一步提供支持。若我们的产品未能达到预期性能,我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com