小鼠VCAM1 ELISA试剂盒(CD106)
Mouse VCAM1 ELISA Kit (CD106)
- Recombinant
- SimpleStep
- 了解详情
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(9 Publications)
Mouse VCAM1 ELISA Kit (CD106) is a single-wash 90-min Simplestep used to quantify Mouse VCAM1 (CD106) with a sensitivity of 5.59 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
查看别名
CD106, Vcam-1, Vcam1, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1
- sELISA
Supplier Data
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)
Example of VCAM1 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)
Example of VCAM1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Project
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)
Mouse VCAM1 (CD106) standard curve comparison data.
Standard curve comparison between mouse VCAM1 (CD106) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)
Titration of mouse unstimulated (-) Lung and L929 extracts within the working range of the assay.
The 1X dilution was an extract load of 250 μg/mL. Interpolated data values (mean +/- SD, n = 2) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)
Titration of mouse serum, citrate plasma, EDTA plasma, and Heparin plasma within the working range of the assay.
The 1X dilution was 0.2% for each biological. Interpolated data values (mean +/- SD, n = 2) are graphed.
反应性数据
产品详情
Mouse VCAM1 ELISA Kit (CD106) (ab201278) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VCAM1 (CD106) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VCAM1 (CD106) with 5.59 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
VCAM1 is important in cell-cell recognition, and appears to function in leukocyte-endothelial cell adhesion. It interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.
Pathways
VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.
靶点信息
文献 (9)
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