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AB201278

小鼠VCAM1 ELISA试剂盒(CD106)

Mouse VCAM1 ELISA Kit (CD106)

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(9 Publications)

Mouse VCAM1 ELISA Kit (CD106) is a single-wash 90-min Simplestep used to quantify Mouse VCAM1 (CD106) with a sensitivity of 5.59 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

查看别名

CD106, Vcam-1, Vcam1, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1

5 Images
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)

Example of VCAM1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)

Example of VCAM1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)
  • sELISA

Project

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)

Mouse VCAM1 (CD106) standard curve comparison data.

Standard curve comparison between mouse VCAM1 (CD106) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)

Titration of mouse unstimulated (-) Lung and L929 extracts within the working range of the assay.

The 1X dilution was an extract load of 250 μg/mL. Interpolated data values (mean +/- SD, n = 2) are graphed.

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)

Titration of mouse serum, citrate plasma, EDTA plasma, and Heparin plasma within the working range of the assay.

The 1X dilution was 0.2% for each biological. Interpolated data values (mean +/- SD, n = 2) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Mouse

检测类型

Sandwich (quantitative)

灵敏度

= 5.59 pg/mL

范围

46.9 - 3000 pg/mL

检测时间

1h 30m

检测平台

Microplate (12 x 8 well strips)

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Mouse VCAM1 ELISA Kit (CD106) (ab201278) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VCAM1 (CD106) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VCAM1 (CD106) with 5.59 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

VCAM1 is important in cell-cell recognition, and appears to function in leukocyte-endothelial cell adhesion. It interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]

回收率

[ { "sample": "Serum", "range": "104 - 109 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "106 - 111 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "107 - 108 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "109 - 113 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "86 - 88 %", "average": "= 87" } ]

规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.

产品实验方案

靶点信息

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.
See full target information Vcam1

文献 (9)

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