小鼠TLR2 ELISA试剂盒(ab224880)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 88 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠TLR2 ELISA试剂盒
参阅全部 TLR2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Cell Extract 5 4.5% 批次间 样品 n Mean SD CV% Cell Extract 3 8.1% -
样品类型
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
88 pg/ml -
范围
312.5 pg/ml - 20000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 94 89% - 98% Cell culture extracts 102 91% - 112% Cell culture media 101 98% - 102% Hep Plasma 89 88% - 91% EDTA Plasma 97 91% - 103% Cit plasma 99 96% - 103% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse
不与反应: Cow -
产品概述
Mouse TLR2 ELISA Kit (ab224880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TLR2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TLR2 with 88 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Toll-Like Receptor 2 (TLR2) is a transmembrane receptor, expressed on a wide variety of cell types including microglia, monocytes, macrophages, and dendritic cells. TLR2 cooperates with multiple other proteins (TLR1, LY96, TLR6, and other) to mediate an immune response to bacterial lipoproteins. Cytokine secretion in response to these bacterium is found to occur in a TLR2 dependent fashion. ab224880 recognizes the extracellular domain of the transmembrane receptor.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml 10X Mouse TLR2 Capture Antibody 1 x 600µl 10X Mouse TLR2 Detector Antibody 1 x 600µl Mouse TLR2 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. -
组织特异性
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
翻译后修饰
Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. -
细胞定位
Membrane. - Information by UniProt
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别名
- CD282
- CD282 antigen
- TIL 4
see all -
数据库链接
- Entrez Gene: 24088 Mouse
- SwissProt: Q9QUN7 Mouse
- Unigene: 87596 Mouse
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224880于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of Mouse TLR2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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Example of Mouse TLR2 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike TLR2 in Mouse serum and plasma samples.The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spike TLR2 in cell culture media sample.The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted sample is 50% cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native TLR2 in Mouse cell and tissue extract samples.The concentrations of TLR2 were measured in duplicate and interpolated from the TLR2 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TLR2 concentration was determined to be 11.1 pg/µg in J774A.1 Cell Extract and 1.75 pg/µg in Spleen Tissue Extract.
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Sandwich ELISA - Mouse TLR2 ELISA Kit (ab224880)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Mouse TLR2 ELISA Kit (ab224880)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Mouse TLR2 ELISA Kit (ab224880)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab224880 被引用在 4 文献中.
- Prasad R et al. Microbial Signatures in The Rodent Eyes With Retinal Dysfunction and Diabetic Retinopathy. Invest Ophthalmol Vis Sci 63:5 (2022). PubMed: 34985498
- Liu J et al. Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism. J Healthc Eng 2022:1473597 (2022). PubMed: 35035808
- von Linstow CU et al. Bone Marrow-Derived IL-1Ra Increases TNF Levels Poststroke. Cells 10:N/A (2021). PubMed: 33924148
- Huang J et al. Hyperactivity of Innate Immunity Triggers Pain via TLR2-IL-33-Mediated Neuroimmune Crosstalk. Cell Rep 33:108233 (2020). PubMed: 33027646