AB213477

小鼠TIM 1 ELISA试剂盒(KIM-1)

Mouse TIM 1 ELISA Kit (KIM-1)

Recombinant
SimpleStep
了解详情

Be the first to review this product! Submit a review

(22 Publications)

Mouse TIM 1 ELISA Kit (KIM-1) is a single-wash 90-min Simplestep used to quantify Mouse TIM 1 (KIM-1) with a sensitivity of 1.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 20 citations

查看别名

CD365, Kim1, Tim1, Timd1, Havcr1, Hepatitis A virus cellular receptor 1 homolog, HAVcr-1, Kidney injury molecule 1, T cell immunoglobulin and mucin domain-containing protein 1, T cell membrane protein 1, T-cell immunoglobulin mucin receptor 1, KIM-1, TIMD-1, TIM-1

13 Images

ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

ELISA

Supplier Data

ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

sELISA

Supplier Data

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Example of mouse KIM-1 standard curve in Sample Diluent 75BP, when applicable.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Example of mouse KIM-1 standard curve in Sample Diluent NS, when applicable.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Interpolated concentrations of native KIM-1 in mouse serum, and plasma samples.

The concentrations of KIM-1 were measured in duplicates, interpolated from the KIM-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 90%, plasma (citrate) 90%, plasma (heparin) 90% and plasma (EDTA) 90%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean KIM-1 concentration was determined to be 170 pg/mL in serum, 125 pg/mL in plasma (citrate), 181 pg/mL in plasma (heparin), and160 pg/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Mouse KIM-1 standard curve comparison data.

Standard curve comparison between mouse KIM-1 SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.

sELISA

Supplier Data

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Example of mouse KIM-1 standard curve in 1X Cell Extraction Buffer PTR, when applicable.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Interpolated concentrations of native mouse KIM-1 in mouse kidney supernatant, urine, and extract based on a 25%, 50%, and 50 μg/mL extract load, respectively.

The concentrations of KIM-1 were measured in duplicate and interpolated from the KIM-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean KIM-1 concentration was determined to be 1,112 pg/mL in neat kidney supernatant, 505 pg/mL in neat urine, and 219 pg/mL in kidney extract, based on a 50 μg/mL extract load.

sELISA

Lab

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Interpolated concentration of native TIM 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 90% mouse serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 75BP.

sELISA

Lab

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Example of mouse TIM 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Interpolated concentration of native TIM 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1,000 µg/mL mouse kidney extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Example of mouse TIM 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Interpolated concentration of native TIM 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% mouse urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Example of mouse TIM 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Mouse

检测类型

Sandwich

灵敏度

= 1.6 pg/mL

范围

6.25 - 400 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

产品详情

Mouse TIM 1 ELISA Kit (KIM-1) ab213477 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse TIM 1 (KIM-1)in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 1.6 pg/mL.

How the assay works

Mouse TIM 1 (KIM-1)SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse TIM 1 ELISA Kit (KIM-1) ab213477 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

KIM-1, also known as Kidney Injury Molecule 1 and Hepatitis A virus cellular receptor 1 homolog (HAVcr-1), or T-cell immunoglobulin and mucin domain 1 (TIM-1) is a 283 amino acid protein that contains an N-terminal immunoglobulin-like domain and is encoded by the HAVCR1 gene. KIM-1 is a single-pass type 1 membrane protein has been identified as a receptor for hepatitis A virus. The TIM gene family participates in host immune response. Urinary KIM-1 levels are elevated in nephropathy and is thought to be a biomarker for renal damage.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]

style

left-column

回收率

[ { "sample": "Serum", "range": "88 - 128 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "96 - 119 %", "average": "= 107" }, { "sample": "Urine", "range": "95 - 112 %", "average": "= 102" }, { "sample": "Tissue Extracts", "range": "90 - 100 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "98 - 112 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "74 - 89 %", "average": "= 82" } ]

style

left-column

规格

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab213477-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB213477-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB213477-CMP15" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB213477-CMP09" }, { "size":"1 x 600 µL", "name":"10X Mouse KIM1 Capture Antibody", "number":"AB213477-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB213477-CMP14" }, { "size":"2 x 1 Vial", "name":"Mouse KIM1 Lyophilized Recombinant Protein", "number":"AB213477-CMP11" }, { "size":"1 x 600 µL", "name":"10X Mouse KIM1 Detector Antibody", "number":"AB213477-CMP07" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB213477-CMP10" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB213477-CMP12" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BP", "number":"AB213477-CMP02" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB213477-CMP13" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB213477-CMP03" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB213477-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213477-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB213477-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB213477-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB213477-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse KIM1 Capture Antibody", "number":"AB213477-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse KIM1 Lyophilized Recombinant Protein", "number":"AB213477-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB213477-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BP", "number":"AB213477-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse KIM1 Detector Antibody", "number":"AB213477-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB213477-CMP12", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB213477-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB213477-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB213477-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB213477-CMP04", "productcode":"" } ] } } }

style

left-column

性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TIM 1 also known as T-cell immunoglobulin and mucin domain 1 is a cell surface protein with a molecular mass of approximately 70 to 80 kDa. It is expressed predominantly in activated T cells and some epithelial cells. TIM 1 plays an important role in immune regulation and can be found at the surface of cells in various tissues including the liver and kidney. This protein can interact with other components which can influence immune response in the host.

Biological function summary

The TIM 1 protein functions as a receptor involved in T cell activity modulation and innate immune response regulation. It is known to bind phosphatidylserine on apoptotic cells which fosters communication between dying cells and phagocytes. TIM 1 does not typically exist as part of a larger complex but its interactions with other molecules is important for its role in the immune system. The protein's activity influences cytokine production and this can affect inflammation processes.

Pathways

TIM 1 is involved in key immune response pathways like the signaling pathways of T cells and the broader adaptive immune response. This protein has been linked with the enhancement of Th2 cytokine production impacting allergic response signaling. It interacts with related proteins like TIM 3 and TIM 4 which together contribute to the fine-tuning of immune responses. Understanding TIM 1 within these pathways helps in grasping its regulatory potential in T-cell mediated immunity.

TIM 1 is implicated in conditions such as asthma and systemic lupus erythematosus (SLE). In asthma improperly regulated Th2 immune responses involve TIM 1 which can exacerbate allergic inflammations. This protein also plays a role in the pathogenesis of SLE a systemic autoimmune disease where TIM 1's regulation of immune tolerance and inflammation is critical. TIM 1's involvement with other proteins like IL-4 and IL-13 in these disorders further highlights its significance in immune dysregulation processes.

style

left-column

产品实验方案

style

left-column

靶点信息

Phosphatidylserine receptor that plays an important functional role in regulatory B-cell homeostasis including generation, expansion and suppressor functions (PubMed : 21821911, PubMed : 25645598, PubMed : 32668241). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses (PubMed : 24703780). Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (PubMed : 24703780). Also regulates expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10 (PubMed : 25582854, PubMed : 25645598). Acts as a regulator of T-cell proliferation (PubMed : 15793576). Confers phagocytic ability on injured kidney epithelial cells, allowing them to bind to and internalize apoptotic bodies and necrotic debris following kidney injury (By similarity). The ectodomain binds to the surface of apoptotic kidney epithelial cells via phosphatidylserine and oxidized phospholipids exposed on the cell membrane of the apoptotic cells (By similarity). HAVCR1-expressing cells are also capable of phagocytosing Gram-negative and Gram-positive bacteria (By similarity).

See full target information Havcr1

style

left-column

文献 (22)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:52 PubMed39875989

2025

VEGFR3 mitigates hypertensive nephropathy by enhancing mitophagy via regulating crotonylation of HSPA1L.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuwen Wu,Jiaxin Fu,Bin Zhu,Wei Meng,Jingyi Ma,Ying Lv,Wenqi Zhao,Fan Wang,Jingjin Liu,Yongshun Wang,Cong Peng,Shuo Zhang

Diabetes, obesity & metabolism 27:40-53 PubMed39344841

2024

Analysing the influence of dapagliflozin on urinary tract infection vulnerability and kidney injury in mice infected with uropathogenic Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Salamon,Sarah Linn-Peirano,Aaron Simoni,Juan de Dios Ruiz-Rosado,Brian Becknell,Preeti John,Laura Schwartz,John David Spencer

Scientific reports 14:16444 PubMed39013979

2024

L-carnitine co-administration prevents colistin-induced mitochondrial permeability transition and reduces the risk of acute kidney injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia L Samodelov,Zhibo Gai,Francesca De Luca,Klara Haldimann,Sven N Hobbie,Daniel Müller,Gerd A Kullak-Ublick,Michele Visentin

Nutrition & diabetes 14:42 PubMed38858392

2024

Vitamin D is involved in the effects of the intestinal flora and its related metabolite TMAO on perirenal fat and kidneys in mice with DKD.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodi Zheng,Yuhong Huang,Mengxue Yang,Lulu Jin,Xuemeng Zhang,Rui Zhang,Yueyue Wu,Cuili Yan,Yuan Gao,Miao Zeng,Fei Li,Xue Zhou,Neng Zhang,Jun Liu,Bingbing Zha

Aging 16:5887-5904 PubMed38517396

2024

Cordyceps sinensis extract protects against acute kidney injury by inhibiting perforin expression in NK cells via the STING/IRF3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Li,Wei Pang,Yuzhu Wang,Yiting Zhang

Environmental toxicology 39:2596-2609 PubMed38205898

2024

Molecular mechanism of circHIPK3 in mitochondrial function in septic acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Tang,Jie Zhang,Jing Han,Danhong Zhang,Hongtao Zhang,Jun Liu,Xiaoyue Li

Frontiers in physiology 14:1240352 PubMed38028812

2023

A clinically-relevant mouse model that displays hemorrhage exacerbates tourniquet-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Balamurugan Packialakshmi,David M Burmeister,Joseph A Anderson,Judah Morgan,Georgetta Cannon,Juliann G Kiang,Yuanyi Feng,Sang Lee,Ian J Stewart,Xiaoming Zhou

JCI insight 8: PubMed36719755

2023

DG9-conjugated morpholino rescues phenotype in SMA mice by reaching the CNS via a subcutaneous administration.

Applications

Unspecified application

Species

Unspecified reactive species

Tejal Aslesh,Esra Erkut,Jun Ren,Kenji Rowel Q Lim,Stanley Woo,Susan Hatlevig,Hong M Moulton,Simon Gosgnach,John Greer,Rika Maruyama,Toshifumi Yokota

Nature communications 14:1223 PubMed36869030

2023

Polynucleotide phosphorylase protects against renal tubular injury via blocking mt-dsRNA-PKR-eIF2α axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Zhu,Mingchao Zhang,Weiran Wang,Shuang Qu,Minghui Liu,Weiwei Rong,Wenwen Yang,Hongwei Liang,Caihong Zeng,Xiaodong Zhu,Limin Li,Zhihong Liu,Ke Zen

Archives of toxicology 96:3349-3361 PubMed36227364

2022

Inhibition of cytochrome P450 enhances the nephro- and hepatotoxicity of ochratoxin A.

Applications

Unspecified application

Species

Unspecified reactive species

Reham Hassan,Daniela González,Zaynab Hobloss,Lisa Brackhagen,Maiju Myllys,Adrian Friebel,Abdel-Latif Seddek,Rosemarie Marchan,Benedikt Cramer,Hans-Ulrich Humpf,Stefan Hoehme,Gisela H Degen,Jan G Hengstler,Ahmed Ghallab

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

小鼠TIM 1 ELISA试剂盒(KIM-1)

Mouse TIM 1 ELISA Kit (KIM-1)

ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

Sandwich ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台

反应性数据

产品详情

精确度

回收率

规格

性能和储存信息

运输条件

推荐的短期储存条件

推荐的长期储存条件

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (22)

搭配使用产品

Assay Kits

Secondary Antibodies

Assay Kits

Primary Antibodies

Abcam Product Promise