小鼠TGF beta 1 ELISA试剂盒(ab119557)
Key features and details
- Sensitivity: 7.8 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠TGF beta 1 ELISA试剂盒
参阅全部 TGF beta 1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4 7.9% 批次间 样品 n Mean SD CV% Overall 4 5.8% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
7.8 pg/ml -
范围
31.3 pg/ml - 2000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 124% - 141% Serum 92% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s TGF beta 1 Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).
Mouse TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Mouse TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mouse TGF beta 1 captured on the plate
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Assay Buffer Concentrate 2 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 6 units Biotinylated mouse anti-TGF beta 1 monoclonal antibody 1 x 120µl Microplate coated with monoclonal antibody to mouse TGF beta 1 (12 x 8 wells) 1 unit Mouse TGF beta 1 Standard lyophilized 2 vials Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究领域
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功能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
组织特异性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
疾病相关
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
序列相似性
Belongs to the TGF-beta family. -
翻译后修饰
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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别名
- Cartilage-inducing factor
- CED
- Differentiation inhibiting factor
see all -
数据库链接
- Entrez Gene: 21803 Mouse
- SwissProt: P04202 Mouse
- Unigene: 248380 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119557于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Sandwich ELISA - TGF beta 1 Mouse ELISA Kit (ab119557)Standard curve of Ms TGFb, with background signal subtracted (duplicates; +/- SD).
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Sandwich ELISA - TGF beta 1 Mouse ELISA Kit (ab119557)Mouse TGFb measured in activated biological fluids, background signal subtracted (duplicates +/- SD). * Samples without activation. -
Typical Standard CurveRepresentative Standard Curve using ab119557.
数据表及文件
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SDS download
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Datasheet download
文献 (60)
ab119557 被引用在 60 文献中.
- Dwivedi V et al. Age associated susceptibility to SARS-CoV-2 infection in the K18-hACE2 transgenic mouse model. Geroscience 46:2901-2913 (2024). PubMed: 38388916
- Hernandez-Espinosa DR et al. Microglial reprogramming by Hv1 antagonism protects neurons from inflammatory and glutamate toxicity. J Neurochem 165:29-54 (2023). PubMed: 36625847
- Wu X et al. Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis. Eur Respir J 61:N/A (2023). PubMed: 36758986
- Landini L et al. Schwann cell insulin-like growth factor receptor type-1 mediates metastatic bone cancer pain in mice. Brain Behav Immun 110:348-364 (2023). PubMed: 36940752
- Hamade DF et al. Release of Interferon-β (IFN-β) from Probiotic Limosilactobacillus reuteri-IFN-β (LR-IFN-β) Mitigates Gastrointestinal Acute Radiation Syndrome (GI-ARS) following Whole Abdominal Irradiation. Cancers (Basel) 15:N/A (2023). PubMed: 36980556