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AB202412

小鼠sTNF RII ELISA试剂盒(TNFRSF1B)

Mouse sTNF RII ELISA Kit (TNFRSF1B)

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Mouse sTNF RII ELISA Kit (TNFRSF1B) is a single-wash 90-min Simplestep used to quantify Mouse sTNF RII (TNFRSF1B) with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

CD120b, Tnfr-2, Tnfr2, Tnfrsf1b, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II

5 Images
Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (AB202412)
  • sELISA

Unknown

Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (AB202412)

Mouse TNFRSF1B standard curve comparison data.

Standard curve comparison between mouse TNFRSF1B SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.

Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (AB202412)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (AB202412)

Interpolated concentrations of sTNF RII in urine and RAW264.7 stimulated cell culture supernatant.

The concentrations of sTNF RII were measured in duplicate and interpolated from the sTNF RII standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 13.3 ng/mL in urine and 3.9 ng/mL in RAW264.7 stimulated cell culture supernatant.

Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (AB202412)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (AB202412)

Interpolated concentrations of sTNF RII in mouse serum, plasma (citrate), and plasma (EDTA).

The concentrations of sTNF RII were measured in duplicate and interpolated from the sTNF RII standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 12 ng/mL in serum, 13.2 ng/mL in plasma (Citrate), and 12.8 ng/mL plasma (EDTA).

Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (AB202412)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (AB202412)

Example of sTNF RII standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (AB202412)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (AB202412)

Comparison of sTNF RII in unstimulated and PMA/PHA-stimulated RAW264.7, L929, and J774A.1 Cells.

Cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. sTNF RII was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated RAW264.7, L929, an J774A.1 and cell culture media. Measured values were interpolated from the sTNF RII Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. sTNF RII was undetectable in media.

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

反应种属

Mouse

检测类型

Sandwich (quantitative)

灵敏度

= 1 pg/mL

范围

6.25 - 400 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Mouse sTNF RII ELISA Kit (TNFRSF1B) (ab202412) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of sTNF RII (TNFRSF1B) protein in cit plasma, edta plasma, serum, urine, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse sTNF RII (TNFRSF1B) with 1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Tumor necrosis factor receptor superfamily member 1B (TNF RII) is a transmembrane protein with an extracellular domain that binds to TNF alpha. This extracellular domain can be proteolytically cleaved to make soluble TNF RII. The standard protein in this kit is soluble TNF RII and the capture and detector antibodies were raised against this region of TNF RII.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]
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回收率

[ { "sample": "Serum", "range": "112 - 129 %", "average": "= 120" }, { "sample": "EDTA Plasma", "range": "110 - 118 %", "average": "= 113" }, { "sample": "Urine", "range": "93 - 113 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "100 - 125 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "97 - 106 %", "average": "= 100" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Soluble TNF Receptor II also known as TNFRSF1B or CD120b acts as a decoy receptor that binds with tumor necrosis factor-alpha (TNF-α) in the serum and extracellular space. It has a molecular mass of approximately 75 kDa. This receptor is expressed in various tissues including the immune cells and plays a role in regulating inflammatory responses. By binding TNF-α Soluble TNF Receptor II prevents TNF-α from interacting with cell surface receptors modulating its inflammatory effects.
Biological function summary

Soluble TNF Receptor II modulates inflammation and immune response. It is a part of the TNF receptor superfamily and can take part in forming a receptor-ligand complex with TNF-α impacting the downstream signaling. This receptor acts to neutralize TNF-α activity by preventing its engagement with pro-inflammatory receptors on cell surfaces. Apart from its inhibitory role it also assists in the clearance of TNF-α from the circulation managing excess TNF-α levels in the body.

Pathways

Soluble TNF Receptor II is dominant in the TNF signaling pathway and the NF-κB pathway. These pathways manage immune response cell survival and apoptosis. TNFR1 another receptor in the TNF family plays a related role in the TNF signaling pathway and often collaborates with Soluble TNF Receptor II to regulate balance between cell death and cell survival. The complex formed by Soluble TNF Receptor II and TNF-α diminishes excessive inflammation by reducing active TNF-α available for binding with TNFR1.

Soluble TNF Receptor II holds significance in rheumatoid arthritis and inflammatory bowel disease. Its presence modifies the course of these diseases by modulating the levels and effects of TNF-α. The inhibition of TNF-α-driven inflammation through interaction with Soluble TNF Receptor II can reduce tissue damage and improve symptoms. In these contexts it shows links with TNF-α which is a well-known mediator of inflammation and plays a role in the pathophysiology of these inflammatory disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity).
See full target information Tnfrsf1b
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文献 (1)

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Diabetes, metabolic syndrome and obesity : targets and therapy 14:4679-4690 PubMed34916815

2021

Astragalus-Saffron-Rhubarb Mixture Delays the Progress of Diabetic Nephropathy in db/db Mice.

Applications

sELISA

Species

Horse

Xiao-Chun Zhou,Yao-Jun Liang,Long Qin,Guo-Hua Wei,Jian-Qin Wang
View all publications
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