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AB309114

小鼠SOST ELISA试剂盒

Mouse SOST ELISA Kit

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Mouse SOST ELISA Kit is a Sandwich ELISA for the measurement of Mouse SOST in Mouse in Cell Culture Media, Biofluids samples.

查看别名

Sclerostin, Sost

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Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)

Interpolated concentrations of mouse SOST in serum, plasma (citrate), and plasma (EDTA)

Interpolated concentration of native SOST was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, plasma (citrate), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)

Example of mouse SOST standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)

Standard curve comparison between mouse SOST SimpleStep ELISA kit (ab309114) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

关键信息

检测方法

Colorimetric

样品类型

Cell culture media, EDTA Plasma, Citrate plasma, Serum

反应种属

Mouse

检测类型

Sandwich

Quantitative
灵敏度

= 2.1 pg/mL

范围

10.938 - 700 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Mouse SOST SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SOST protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Mouse SOST with 2.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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回收率

[ { "sample": "Serum", "range": "83 - 98 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "87 - 103 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "91 - 109 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "83 - 110 %", "average": "= 95" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309114-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB309114-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309114-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309114-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309114-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309114-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309114-CMP06", "productcode":"" }, { "size":"1 x 6 Vial", "name":"Antibody Diluent CPI2", "number":"AB309114-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse SOST Lyophilized Recombinant Protein", "number":"AB309114-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SOST Capture Antibody", "number":"AB309114-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SOST Detector Antibody", "number":"AB309114-CMP02", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
See full target information Sost
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websiteProtocolBooklet
en
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