AB195459

小鼠Retinol binding蛋白4 ELISA试剂盒(RBP4)

Name: 小鼠Retinol binding protein 4 ELISA试剂盒 (RBP4) (ab195459)| Abcam中文官网
Brand: Abcam Limited
SKU: AB195459
Price: 7592 CNY
Availability: InStock

Mouse Retinol binding protein 4 ELISA Kit (RBP4)

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Mouse Retinol binding protein 4 ELISA Kit (RBP4) is a Sandwich ELISA for the measurement of Mouse Retinol binding protein 4 (RBP4) in Mouse in Biofluids samples.

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Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP, Rbp4

1 Images

Sandwich ELISA - Mouse Retinol binding protein 4 ELISA Kit (RBP4) (AB195459)

sELISA

Supplier Data

Sandwich ELISA - Mouse Retinol binding protein 4 ELISA Kit (RBP4) (AB195459)

Representative standard curve using ab195459.

关键信息

检测方法

Colorimetric

样品类型

Urine, Plasma, Serum

反应种属

Mouse

检测类型

Sandwich

灵敏度

= 0.4 ng/mL

范围

0.625 - 40 ng/mL

检测时间

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's Retinol binding protein 4 (RBP4) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RBP4 concentrations in mouse plasma, serum and urine samples.

An RBP4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an RBP4 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of RBP4 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab195459-1x96Tests", "assetComponentDetails": [ { "size":"1 x 120 µL", "name":"50X Biotinylated Mouse RBP4 Antibody", "number":"AB195459-CMP10", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB195459-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse RBP4 Standard (Lyophilised)", "number":"AB195459-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB195459-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB195459-CMP08", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB195459-CMP07", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB195459-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Mouse RBP4 Microplate (12 x 8 well strips)", "number":"AB195459-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB195459-CMP01", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

Please refer to protocols

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RBP4 or retinol-binding protein 4 is a carrier protein that is essential for the transport of retinol or vitamin A in the bloodstream. Also known by the names RBP or plasma retinol-binding protein this protein has a molecular weight of approximately 21 kDa. RBP4 is mainly expressed in the liver and secreted into the blood where it binds to retinol and protects it from oxidation. The structure of RBP4 allows it to fit into binding pockets facilitating the transport of retinol to peripheral tissues.

Biological function summary

RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.

Pathways

RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.

RBP4 is linked to the development of insulin resistance and type 2 diabetes. Elevated RBP4 levels correlate with these conditions and highlight its role in metabolic processes. Studies also show a connection between altered RBP4 levels and obesity with insulin resistance often occurring in conjunction with obesity. The protein is implicated in these metabolic disorders through its interactions with insulin-regulating proteins which influence glucose homeostasis.

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产品实验方案

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靶点信息

Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.

See full target information Retinol-binding protein 4

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websiteProtocolBooklet

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小鼠Retinol binding蛋白4 ELISA试剂盒(RBP4)

Mouse Retinol binding protein 4 ELISA Kit (RBP4)

Sandwich ELISA - Mouse Retinol binding protein 4 ELISA Kit (RBP4) (AB195459)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台