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AB108900

小鼠Resistin ELISA试剂盒(RETN)

Mouse Resistin ELISA kit (RETN)

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Mouse Resistin ELISA kit (RETN) is a Sandwich ELISA for the measurement of Mouse Resistin (RETN) in Mouse in Biofluids, Cell Culture Media samples.

查看别名

Fizz3, Retn, Resistin, Adipose tissue-specific secretory factor, Adipose-specific cysteine-rich secreted protein A12-alpha, Cysteine-rich secreted protein FIZZ3, ADSF

2 Images
Sandwich ELISA - Mouse Resistin ELISA kit (RETN) (AB108900)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Resistin ELISA kit (RETN) (AB108900)

Representative Standard Curve using ab108900

Sandwich ELISA - Mouse Resistin ELISA kit (RETN) (AB108900)
  • sELISA

Unknown

Sandwich ELISA - Mouse Resistin ELISA kit (RETN) (AB108900)

Representative Standard Curve using ab108900

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Mouse

检测类型

Sandwich

Quantitative
灵敏度

= 21 pg/mL

检测时间

5h

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Abcam's Resistin (RETN) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Resistin levels in mouse plasma, serum and cell culture supernatant samples.

A Resistin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Resistin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108900-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108900-CMP11", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108900-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Resistin Microplate (12 x 8 well strips)", "number":"AB108900-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108900-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108900-CMP03", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108900-CMP04", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB108900-CMP10", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108900-CMP09", "productcode":"" }, { "size":"1 x 140 µL", "name":"50X Biotinylated Mouse Resistin Antibody", "number":"AB108900-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Resistin Standard", "number":"AB108900-CMP06", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
Multi
储存信息
Please refer to protocols
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Resistin also known as FIZZ3 or ADSF is a protein with a mass of approximately 12.5 kDa. It is mainly expressed in adipose tissue and secreted by adipocytes. In human beings it is also found in inflammatory zones like macrophages and the placenta. Resistin functions as a signaling molecule or cytokine which plays roles in inflammation and metabolism.
Biological function summary

Resistin affects glucose homeostasis and has been shown to modulate insulin resistance. It does not function as part of a larger protein complex but instead acts through specific receptors on cell surfaces. Resistin influences several cellular processes including energy balance and the modulation of inflammatory responses.

Pathways

Several processes contribute to the detailed function of resistin. It is involved in the insulin signaling pathway and the inflammatory pathway. Resistin interacts with proteins and receptors like TLR4 which have key roles in inflammatory response signaling and metabolic regulation. These interactions can disrupt insulin signaling and glucose uptake in many cell types.

Resistin has connections to type 2 diabetes and obesity. Its role in insulin resistance makes it a significant molecule in the pathophysiology of type 2 diabetes. Additionally resistin can influence obesity through its effect on energy balance and fat distribution. Resistin's relationship with proteins like TNF-alpha and IL-6 further emphasizes its role in linking metabolic syndrome with chronic inflammatory states.
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产品实验方案

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靶点信息

Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
See full target information Retn
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websiteProtocolBooklet
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