小鼠/大鼠TPA ELISA试剂盒
Mouse/Rat TPA ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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Mouse/Rat TPA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse/Rat TPA with a sensitivity of 4.98 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, Plat
- sELISA
Supplier Data
Sandwich ELISA - Mouse/Rat TPA ELISA Kit (AB233615)
Interpolated concentrations of native tPA in rat serum, plasma and urine samples.
The concentrations of tPA were measured in duplicates, interpolated from the tPA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 12.5%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean tPA concentration in the neat samples was determined to be 1,657 pg/mL in serum, 5,687 pg/mL in plasma (citrate), and 388 pg/mL in urine.
- sELISA
Supplier Data
Sandwich ELISA - Mouse/Rat TPA ELISA Kit (AB233615)
Interpolated concentrations of native tPA in mouse and rat serum, plasma and urine samples.
The concentrations of tPA were measured in duplicates, interpolated from the tPA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 12.5%, plasma (EDTA) 12.5%, plasma (citrate) 12.5%, and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean tPA concentration in the neat samples was determined to be 540 pg/mL in serum, 2,567 pg/mL in plasma (heparin), 1,971 pg/mL in plasma (EDTA), 3,141 pg/mL in plasma (citrate), and 2,223 pg/mL in urine.
- sELISA
Supplier Data
Sandwich ELISA - Mouse/Rat TPA ELISA Kit (AB233615)
Example of mouse and rat tPA standard curve in Sample Diluent NS.
The tPA standard curve was prepared as described in Section 10.
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反应性数据
产品详情
Mouse/Rat TPA ELISA Kit (ab233615) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TPA protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat TPA with 4.98 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
The standard is affinity purified.
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补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.
Pathways
TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.
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