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AB214572

小鼠Prolactin/PRL ELISA试剂盒

Mouse Prolactin/PRL ELISA Kit

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(3 Publications)

Mouse Prolactin/PRL ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Prolactin/PRL with a sensitivity of 139 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Decidual prolactin, GHA1, Growth hormone A1, Lactogenic Hormone, Luteotropic Hormone, Mammotropin, Prolactin, Prolactin precursor

5 Images
Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

Comparison of native Prolactin concentrations in pregnant and normal mouse serum.

The concentrations of Prolactin were measured in three 2-fold serial dilutions starting at 25% serum samples in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Prolactin per mL of neat serum (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 6.5 ng/mL in pregnant mouse serum and 2.6 ng/mL in normal mouse serum.

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

Interpolated concentrations of spike Prolactin in normal mouse serum samples.

The concentrations of Prolactin were measured in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. Undiluted samples are as follows : normal mouse serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

Example of mouse Prolactin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

Interpolated concentrations of native Prolactin in mouse pituitary cell extract based on a 250 ng/mL extract load.

The concentrations of Prolactin were measured in duplicate and interpolated from the Prolactin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 20 ng/mL in mouse pituitary cell extract.

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

Example of mouse Prolactin standard curve in1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture extracts, Tissue Extracts, Serum

反应种属

Mouse

检测类型

Sandwich

Quantitative
灵敏度

= 139 pg/mL

范围

0.562 - 36 ng/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

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Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

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  • 92
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Mouse Prolactin/PRL ELISA Kit (ab214572) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Prolactin/PRL protein in cell culture extracts, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Prolactin/PRL with 139 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Prolactin/PRL is a secreted neuroendocrine pituitary hormone. Prolactin acts primarily on the mammary gland by promoting lactation, but has also additional pleiotropic effects both in males and females. Mature prolactin is a 199 amino acid long secreted monomeric protein. Prolactin can be also found in less biologically active form, macroprolactin, in a complex with IgG.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]
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回收率

[ { "sample": "Serum", "range": "69 - 80 %", "average": "= 75" }, { "sample": "Tissue Extracts", "range": "98 - 109 %", "average": "= 102" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab214572-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB214572-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB214572-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB214572-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB214572-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB214572-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB214572-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB214572-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB214572-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB214572-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Prolactin Lyophilized Recombinant Protein", "number":"AB214572-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Prolactin Capture Antibody", "number":"AB214572-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Prolactin Detector Antibody", "number":"AB214572-CMP03", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin (PRL) also known as mammotropin is a peptide hormone with a molecular mass of approximately 23 kDa. PRL is primarily expressed in the anterior pituitary gland but can also be found in other tissues such as the mammary glands brain and immune system. PRL plays an important role in lactation stimulating milk production in mammals. The PRL protein functions by binding to the prolactin receptor a member of the cytokine receptor family initiating signal transduction pathways.
Biological function summary

PRL influences a variety of physiological processes including reproduction metabolism and osmoregulation. It acts as a single entity rather than part of a larger protein complex affecting development and growth through its regulatory activities. In addition to lactation PRL also plays roles in the immune system cell proliferation and the production of surfactant in the lungs.

Pathways

Prolactin is deeply involved in the JAK-STAT signaling pathway and the MAPK/ERK pathway which are pivotal for transmitting signals from the prolactin receptor to the nucleus. Through these pathways PRL interacts with proteins like Janus kinase (JAK) and Signal Transducer and Activator of Transcription (STAT) proteins facilitating its wide range of biological effects. This interplay helps regulate gene expression critical for various physiological conditions.

PRL is linked to conditions such as hyperprolactinemia and prolactinoma. Hyperprolactinemia characterized by elevated levels of prolactin in the blood can lead to symptoms such as galactorrhea and infertility. In the context of prolactinoma an adenoma of the pituitary gland PRL's overproduction is common. These disorders often relate to proteins like dopamine which typically acts to inhibit PRL secretion from the pituitary gland and any disruption in this regulation can result in the aforementioned conditions.
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产品实验方案

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靶点信息

See full target information Prolactin/PRL
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Basic and clinical andrology 35:9 PubMed40055615

2025

Pelvic vasectomy and its protective effects on rat testis function.

Applications

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Species

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Heng Yang,Yujun Chen,Xiaofeng Cheng,Jingxin Wu,Ruohui Huang,Biao Qian,Gongxian Wang

Journal of neuroinflammation 21:302 PubMed39563331

2024

Microglia-derived ADAM9 promote GHRH neurons pyroptosis by Mad2L2-JNK-caspase-1 pathway in subarachnoid hemorrhage.

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Jian Mao,Yun Bao,Fan Liu,Qiyun Ye,Junxiang Peng,Jing Nie,Lijun Huang,Yonghong Liao,Yiheng Xing,Dongyang Wu,Ke Wang,Wenfeng Feng,Songtao Qi,Jun Pan,Binghui Qiu

Cell death & disease 12:879 PubMed34564702

2021

Satb2 regulates the development of dopaminergic neurons in the arcuate nucleus by Dlx1.

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Qiong Zhang,Lei Zhang,Ying Huang,Pengcheng Ma,Bingyu Mao,Yu-Qiang Ding,Ning-Ning Song
View all publications
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