小鼠Osteoprotegerin ELISA试剂盒(TNFRSF11B)
Mouse Osteoprotegerin ELISA Kit (TNFRSF11B)
- Recombinant
- SimpleStep
- 了解详情
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(3 Publications)
Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) is a single-wash 90-min Simplestep used to quantify Mouse Osteoprotegerin (TNFRSF11B) with a sensitivity of 5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
Ocif, Opg, Tnfrsf11b, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin
- sELISA
Supplier Data
Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)
Interpolated concentrations of Osteoprotegerin in mouse lung day 5 supernatant, serum and plasma (heparin) samples.
The concentrations of Osteoprotegerin were measured in duplicates, interpolated from the Osteoprotegerin standard curve and corrected for sample dilution. Note that 1X Diluted samples were neat. The interpolated, dilution factor-corrected values are plotted in pg of Osteoprotegerin per mL of neat sample (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)
Example of Osteoprotegerin standard curve for prepared in Sample Diluent 50BS measurements.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)
Mouse Osteoprotegerin standard curve comparison data.
Standard curve comparison between mouse Osteoprotegerin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)
Example of Osteoprotegerin standard curve for prepared in Sample Diluent NS measurements.
Background-subtracted data values (mean +/- SD) are graphed.
搭配使用产品
反应性数据
产品详情
Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (ab203365) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteoprotegerin (TNFRSF11B) protein in cell culture supernatant, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Osteoprotegerin (TNFRSF11B) with 5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Osteoprotegerin acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Osteoprotegerin inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. Osteoprotegerin may also play a role in preventing arterial calcification. Osteoprotegerin may act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
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性能和储存信息
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储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
OPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.
Pathways
OPG operates within the RANK/RANKL/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.
靶点信息
文献 (3)
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Nature communications 13:4820 PubMed35973996
2022
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Nature communications 13:771 PubMed35140213
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Nature communications 11:431 PubMed31969567
2020
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