小鼠Myeloperoxidase ELISA试剂盒(MPO) (ab275109)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 117 pg/ml
- Range: 273.44 pg/ml - 17500 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Myeloperoxidase ELISA试剂盒(MPO)
参阅全部 Myeloperoxidase 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 3% 批次间 样品 n Mean SD CV% Serum 3 6% -
样品类型
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
117 pg/ml -
范围
273.44 pg/ml - 17500 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 97 74% - 110% Cell culture media 99 96% - 100% Hep Plasma 115 108% - 120% EDTA Plasma 108 102% - 118% Cit plasma 119 110% - 126% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse -
产品概述
Mouse Myeloperoxidase ELISA kit (ab275109) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Myeloperoxidase protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Myeloperoxidase with 117 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Myeloperoxidase (MPO) is part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. MPO is a hemoprotein expressed in the neutrophils (polymorphonuclear leukocytes) and is secreted during activation. Myeloperoxidase catalyzes the oxidation of chloride ions to hypochlorus acid, which is a potent antimicrobial agent. Myeloperoxidase pro- oxidative and pro-inflammatory properties cause an increase in MPO in multiple diseases including heart disease, myocardial infraction, and multiple sclerosis. Myeloperoxidase is a clinical marker of myocardial infraction and cardiovascular disease.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse Myeloperoxidase (MPO) Capture Antibody 1 x 600µl 10X Mouse Myeloperoxidase (MPO) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR2 1 x 6ml Mouse Myeloperoxidase (MPO) Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
疾病相关
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
序列相似性
Belongs to the peroxidase family. XPO subfamily. -
细胞定位
Lysosome. - Information by UniProt
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别名
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
see all -
数据库链接
- Entrez Gene: 17523 Mouse
- SwissProt: P11247 Mouse
- Unigene: 4668 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab275109于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of mouse Myeloperoxidase standard curve in Sample Diluent NS.
The Myeloperoxidase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Myeloperoxidase in mouse serum and plasma samples.The concentrations of Myeloperoxidase were measured in duplicates, interpolated from the Myeloperoxidase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 3.8%, plasma (EDTA) 7.5% and plasma (heparin) 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myeloperoxidase concentration was determined to be 138 ng/mL in neat serum, 244 ng/mL in plasma (citrate) and 124 ng/mL in neat plasma (EDTA) and 123 ng/mL in neat plasma (heparin).
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Sandwich ELISA - Mouse Myeloperoxidase ELISA Kit (MPO) (ab275109)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Mouse Myeloperoxidase ELISA Kit (MPO) (ab275109)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Mouse Myeloperoxidase ELISA Kit (MPO) (ab275109)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab275109 被引用在 4 文献中.
- Guo JG et al. MicroRNA-155-5p inhibition alleviates irritable bowel syndrome by increasing claudin-1 and ZO-1 expression. Ann Transl Med 11:34 (2023). PubMed: 36819593
- Obermüller B et al. Probiotic OMNi-BiOTiC® 10 AAD Reduces Cyclophosphamide-Induced Inflammation and Adipose Tissue Wasting in Mice. Nutrients 15:N/A (2023). PubMed: 37630845
- Peng C et al. Early life administration of Bifidobacterium bifidum BD-1 alleviates long-term colitis by remodeling the gut microbiota and promoting intestinal barrier development. Front Microbiol 13:916824 (2022). PubMed: 35935215
- Li Z et al. Peptide YY 3-36 attenuates trinitrobenzene sulfonic acid-induced colitis in mice by modulating Th1/Th2 differentiation. Bioengineered 13:10144-10158 (2022). PubMed: 35443853