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AB100730

小鼠MMP2 ELISA试剂盒

Mouse MMP2 ELISA Kit

3

(1 Review)

|

(4 Publications)

Mouse MMP2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse MMP2 in Mouse in Biofluids, Cell Culture Media samples.

查看别名

72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, Mmp2

2 Images
Sandwich ELISA - Mouse MMP2 ELISA Kit (AB100730)
  • sELISA

Lab

Sandwich ELISA - Mouse MMP2 ELISA Kit (AB100730)

MMP2 measured in biological fluids showing quantity (pg) per mL tested sample

Sandwich ELISA - Mouse MMP2 ELISA Kit (AB100730)
  • sELISA

Unknown

Sandwich ELISA - Mouse MMP2 ELISA Kit (AB100730)

Representative standard curve using ab100730

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Mouse

检测类型

Sandwich (quantitative)

灵敏度

< 25 pg/mL

范围

27.43 - 20000 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Abcam's MMP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse MMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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回收率

[ { "sample": "Cell culture supernatant", "range": "86 - 104 %", "average": "= 94.21" }, { "sample": "Serum", "range": "96 - 127 %", "average": "= 107.6" }, { "sample": "Plasma", "range": "95 - 120 %", "average": "= 105.6" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100730-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100730-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100730-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100730-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse MMP2", "number":"AB100730-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100730-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse MMP2 Standard (lyophilized)", "number":"AB100730-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100730-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MMP2 Microplate (12 x 8 wells)", "number":"AB100730-CMP06", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.
Biological function summary

Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.

Pathways

MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.

Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.
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产品实验方案

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靶点信息

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity).. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels (By similarity).. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
See full target information Mmp2
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology and pharmacology 42:63-8 PubMed26773360

2016

Exposure to fipronil elevates systolic blood pressure and disturbs related biomarkers in plasma of rats.

Applications

Unspecified application

Species

Unspecified reactive species

Joao Leandro Chaguri,Antonio Francisco Godinho,Daniel França Horta,Victor Hugo Gonçalves-Rizzi,Jose Sergio Possomato-Vieira,Regina Aparecida Nascimento,Carlos Alan Dias-Junior

International journal of oral science 7:220-31 PubMed26674424

2015

The effects of interleukin-1β in modulating osteoclast-conditioned medium's influence on gelatinases in chondrocytes through mitogen-activated protein kinases.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xie,Na Fu,Lin-Yi Cai,Tao Gong,Guo Li,Qiang Peng,Xiao-Xiao Cai

Basic & clinical pharmacology & toxicology 116:508-15 PubMed25308714

2014

Metalloproteinase Inhibition Protects against Reductions in Circulating Adrenomedullin during Lead-induced Acute Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Regina A Nascimento,Gabryella Mendes,Jose S Possomato-Vieira,Victor Hugo Gonçalves-Rizzi,Hélio Kushima,Flavia K Delella,Carlos A Dias-Junior

The Journal of biological chemistry 289:32030-43 PubMed25271168

2014

Global ablation of the mouse Rab11a gene impairs early embryogenesis and matrix metalloproteinase secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyan Yu,Ghassan Yehia,Juanfei Wang,Ewa Stypulkowski,Ryotaro Sakamori,Ping Jiang,Berenice Hernandez-Enriquez,Tracy S Tran,Edward M Bonder,Wei Guo,Nan Gao
View all publications
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websiteProtocolBooklet
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