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AB327296

Mouse MMP-2 ELISA Kit, Chemiluminescent

Mouse MMP-2 ELISA Kit, Chemiluminescent

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Mouse MMP-2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse MMP-2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 45.27 - 33000 pg/ml with a sensitivity of 9.71 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

查看别名

72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, Mmp2

3 Images
Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)
  • sELISA

Unknown

Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)

Interpolated concentrations of native MMP-2 in mouse serum and plasma samples.

The concentrations of MMP-2 were measured in duplicates interpolated from the MMP-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 0.3% plasma (EDTA) 0.3% and plasma (heparin) 0.3%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean MMP-2 concentration was determined to be 824.05 ng/mL in serum 737.92 ng/mL in plasma (EDTA) 884.77 ng/mL in plasma (citrate).

Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)
  • sELISA

Lab

Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)

Standard curve comparison between the Chemiluminescent Mouse MMP-2 SimpleStep ELISA and colorimetric Mouse MMP-2 SimpleStep ELISA (ab254516) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)
  • sELISA

Lab

Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)

Dynamic range comparison between the Chemiluminescent Mouse MMP-2 SimpleStep ELISA, colorimetric Mouse MMP-2 SimpleStep ELISA (ab254516), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

关键信息

检测方法

Luminescent

样品类型

Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma

反应种属

Human, Mouse, Rat, Monkey, Cow

检测类型

Sandwich

Quantitative
灵敏度

= 9.71 pg/mL

范围

45.27 - 33000 pg/mL

检测时间

55m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Mouse MMP-2 ELISA Kit, Chemiluminescent (ab327296) is a rapid single-wash sandwich 55-min ELISA to measure Mouse MMP-2 in Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma with a sensitivity of 9.71 pg/mL.

How the assay works

Mouse MMP-2 ELISA Kit, Chemiluminescent (ab327296) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse MMP-2 (ab327296) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free (Capture) ab259743
- Anti-MMP2 antibody [EPR22283-37] - BSA and Azide free (Detector) ab259744
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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "87 - 108 %", "average": "= 95" }, { "sample": "Serum", "range": "83 - 106 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "80 - 118 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "85 - 113 %", "average": "= 95" } ]
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规格

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab327296-10x96Tests", "assetComponentDetails": [ { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327296-CMP06" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB327296-CMP10" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327296-CMP09" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB327296-CMP04" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent B", "number":"AB327296-CMP08" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent A", "number":"AB327296-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327296-CMP05" }, { "size":"10 x 600 µL", "name":"10X Mouse MMP-2 Detector Antibody", "number":"AB327296-CMP02" }, { "size":"10 x 1 Vial", "name":"Mouse MMP-2 Lyophilized Recombinant Protein", "number":"AB327296-CMP03" }, { "size":"10 x 600 µL", "name":"10X Mouse MMP-2 Capture Antibody", "number":"AB327296-CMP01" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab327296-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB327296-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MMP-2 Capture Antibody", "number":"AB327296-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327296-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327296-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327296-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB327296-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MMP-2 Detector Antibody", "number":"AB327296-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB327296-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse MMP-2 Lyophilized Recombinant Protein", "number":"AB327296-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB327296-CMP07", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity).. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels (By similarity).. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
See full target information Mmp2
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websiteProtocolBooklet
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