小鼠MMP-2 ELISA试剂盒
Mouse MMP-2 ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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(4 Publications)
Mouse MMP-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MMP-2 with a sensitivity of 26.91 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, Mmp2
- sELISA
Unknown
Sandwich ELISA - Mouse MMP-2 ELISA Kit (AB254516)
Interpolated concentrations of native MMP-2 in mouse serum and plasma samples.
The concentrations of MMP-2 were measured in duplicates, interpolated from the MMP-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 0.3%, plasma (EDTA) 0.3% and plasma (heparin) 0.3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-2 concentration was determined to be 824.05 ng/mL in serum, 737.92 ng/mL in plasma (EDTA), 884.77 ng/mL in plasma (citrate).
- sELISA
Supplier Data
Sandwich ELISA - Mouse MMP-2 ELISA Kit (AB254516)
Example of mouse MMP-2 standard curve in Sample Diluent NS.
The MMP-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse MMP-2 ELISA Kit (AB254516)
Mouse MMP-2 standard curve comparison.
Standard Curve comparison between Mouse MMP-2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
反应性数据
产品详情
Mouse MMP-2 ELISA Kit (ab254516) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP-2 protein in cell culture supernatant, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MMP-2 with 26.91 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse MMP-2 (matrix metalloproteinase 2) is a 72 kD protein that is a part of the matrix metalloproteinases (MMPs) family consisting of 24 known human zinc proteases. MMPs essential role is breaking down components of the extracellular matrix. Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell adhesion molecules, and other proteinases. Most MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. MMPs are synthesized as pro-enzymes and secreted before conversion to their active form. MMP activity is regulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases. Mouse MMP-2 shares 96%, 99%, 95%, and 96% sequence homology with human, rat, cow, and monkey respectively.
精确度
回收率
规格
性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.
Pathways
MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.
靶点信息
文献 (4)
Recent publications for all applications. Explore the full list and refine your search
Cells 13: PubMed39273042
2024
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TH open : companion journal to thrombosis and haemostasis 7:e1-e13 PubMed36751299
2023
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Folia neuropathologica 59:359-371 PubMed35114776
2022
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Autophagy 17:4062-4082 PubMed33834930
2021
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