AB246547

小鼠MCP-5 ELISA试剂盒

Mouse MCP-5 ELISA Kit

Recombinant
SimpleStep
了解详情

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(1 Publication)

Mouse MCP-5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MCP-5 with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Mcp5, Scya12, Ccl12, C-C motif chemokine 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Small-inducible cytokine A12, MCP-5

2 Images

Sandwich ELISA - Mouse MCP-5 ELISA Kit (AB246547)

sELISA

Supplier Data

Sandwich ELISA - Mouse MCP-5 ELISA Kit (AB246547)

Interpolated concentrations of spiked MCP-5 in mouse serum, plasma (citrate) and lung supernatant samples.

The concentrations of MCP-5 were measured in duplicates and interpolated from the MCP-5 standard curves. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, and lung supernatant 37.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse MCP-5 ELISA Kit (AB246547)

Example of mouse MCP-5 standard curve in Sample Diluent NS.

The MCP-5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Citrate plasma, Cell culture supernatant, Serum

反应种属

Mouse

检测类型

Sandwich (quantitative)

灵敏度

= 6 pg/mL

范围

27.56 - 1700 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Mouse MCP-5 ELISA Kit (ab246547) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP-5 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MCP-5 with 6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]

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回收率

[ { "sample": "Cell culture supernatant", "range": "106 - 114 %", "average": "= 111" }, { "sample": "Serum", "range": "113 - 119 %", "average": "= 116" }, { "sample": "Citrate plasma", "range": "100 - 106 %", "average": "= 103" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246547-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246547-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246547-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246547-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246547-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB246547-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse MCP-5 Lyophilized Recombinant Protein", "number":"AB246547-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MCP-5 Capture Antibody", "number":"AB246547-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB246547-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MCP-5 Detector Antibody", "number":"AB246547-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246547-CMP06", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCP-5 also known as CCL12 is a chemokine protein that plays a role in immune response by recruiting and activating leukocytes. It has a molecular mass of approximately 12 kDa. This protein is expressed mainly in tissues related to the immune system including the spleen and liver. MCP-5 exhibits a sequence homology with other members of the C-C chemokine family and shares functional similarities with MCP-1.

Biological function summary

MCP-5 takes part in promoting the migration of monocytes and lymphocytes to sites of inflammation. This chemokine is not a part of a complex but interacts with specific receptors on target cells such as CCR2. Through these interactions MCP-5 helps mediate a range of immune responses enhancing both innate and adaptive immune activities. It also contributes to the clearance of pathogens by guiding leukocytes to infection sites.

Pathways

MCP-5 plays an important role in the chemokine signaling pathway and the inflammatory response pathway. Through chemokine signaling MCP-5 interacts with CCR2 facilitating the movement of immune cells to areas of tissue damage or infection. This regulation of cell traffic is essential for maintaining homeostasis and responding to pathological changes in tissues. Another protein connected to MCP-5 in these pathways is MCP-3 which shares overlapping functions in immune cell recruitment.

MCP-5 has been implicated in conditions such as asthma and rheumatoid arthritis. In these disorders MCP-5 contributes to the recruitment of inflammatory cells worsening symptoms. It also shows a connection with other proteins like MCP-1 which similarly aids in the movement of monocytes to inflammatory sites. The presence and activity of MCP-5 and its related proteins can therefore represent potential targets for therapeutic interventions in controlling excessive inflammatory responses.

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产品实验方案

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靶点信息

Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

See full target information Ccl12

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文献 (1)

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Oxidative medicine and cellular longevity 2021:9987219 PubMed34484568

2021

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Applications

Unspecified application

Species

Unspecified reactive species

Jian-An Pan,Hao Lin,Jian-Ying Yu,Hui-Li Zhang,Jun-Feng Zhang,Chang-Qian Wang,Jun Gu

View all publications

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websiteProtocolBooklet

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小鼠MCP-5 ELISA试剂盒

Mouse MCP-5 ELISA Kit

Sandwich ELISA - Mouse MCP-5 ELISA Kit (AB246547)

Sandwich ELISA - Mouse MCP-5 ELISA Kit (AB246547)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台