小鼠MASPIN ELISA试剂盒
Mouse MASPIN ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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Mouse MASPIN ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MASPIN with a sensitivity of 18.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Pi5, Spi5, Spi7, Serpinb5, Serpin B5, Maspin, Peptidase inhibitor 5, PI-5
- sELISA
Supplier Data
Sandwich ELISA - Mouse MASPIN ELISA Kit (AB238270)
Example of mouse MASPIN standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse MASPIN ELISA Kit (AB238270)
Interpolated concentrations of native MASPIN in mouse skin extract and mouse small intestine extract based on a 5 μg/mL and 1,500 μg/mL extract load, respectively. The concentrations of MASPIN were measured in duplicate and interpolated from the MASPIN standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MASPIN concentration was determined to be 1,060.3 pg/mL in mouse skin extract and 2,199.6 pg/mL in mouse small intestine extract.
搭配使用产品
反应性数据
产品详情
Mouse MASPIN ELISA Kit (ab238270) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MASPIN protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MASPIN with 18.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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性能和储存信息
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储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MASPIN influences apoptosis and cell motility playing a critical role in tumor suppression. It does not form part of a complex but interacts directly with extracellular matrix components. MASPIN can inhibit angiogenesis by reducing the proliferation and migration of endothelial cells. Scientists have noted its role in maintaining normal cell architecture and inhibiting tumor cell invasion.
Pathways
Researchers place MASPIN in important signaling networks impacting cancer progression and metastasis. It participates in the PI3K/AKT pathway thereby influencing cell growth and survival. MASPIN also intersects with TGF-β signaling and related proteins like SMAD which mediate growth arrest and apoptosis. By altering these pathways MASPIN helps modulate responses to cellular stress and inhibit lethal growth in cancer cells.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com