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小鼠Leptin ELISA试剂盒(ab108881)

Submit a review Q&A (1)References (1)
Typical Standard Curve

    Key features and details

    • Sensitivity: 0.35 ng/ml
    • Range: 0.75 ng/ml - 48 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    概述

    • 产品名称

      小鼠Leptin ELISA试剂盒
      参阅全部 Leptin 试剂盒

    • 检测方法

      Colorimetric

    • 精确度

      批次内
      样品 n Mean SD CV%
      Overall 5.6%
      批次间
      样品 n Mean SD CV%
      Overall 9.8%

    • 样品类型

      Cell culture supernatant, Serum, Plasma

    • 检测类型

      Sandwich (quantitative)

    • 灵敏度

      = 0.35 ng/ml

    • 范围

      0.75 ng/ml - 48 ng/ml

    • 检测时间

      5h 00m

    • 实验步骤

      Multiple steps standard assay

    • 种属反应性

      与反应: Mouse

    • 产品概述

      Abcam’s Leptin mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of leptin levels in plasma, serum and cell culture supernatants.


      A Leptin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Leptin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leptin captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • 经测试应用

      适用于: Sandwich ELISAmore details

    • 平台

      Microplate

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab108881于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Sandwich ELISA
    Use at an assay dependent concentration.
    说明
    Sandwich ELISA
    Use at an assay dependent concentration.

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108881

    文献 (1)

    发表研究结果有使用 ab108881？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab108881 被引用在 1 文献中.

    • Cui XB  et al. RGC32 deficiency protects against high-fat diet-induced obesity and insulin resistance in mice. J Endocrinol 224:127-37 (2015). Functional Studies ; Mouse . PubMed: 25385871

    客户评价及客户问答

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    Question

    I would like to measure Leptin in either plasma or serum of pigs. I would like to know the chances of one of the abcam available leptin ELISA kits to work in pigs. Find below the pig leptin aminoacid sequence
    MRCGPLCRFLWLWPYLSYVEAVPIWRVQDDTKTLIKTIVTRISDISHMQSVSSKQRVTGL
    DFIPGLHPVLSLSKMDQTLAIYQQILTSLPSRNVIQISNDLENLRDLLHLLASSKSCPLP
    QARALETLESLGGVLEASLYSTEVVALSRLQGALQDMLRQLDLSPGC
    Thank you

    Read More
    Answer

    Thank you very much for your inquiry and your interest in our kits.

    Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

    For the other three kits, ab100581, ab100718 and ab100773,the laboratory has not tested these on pig samples and no prediction can be made, as not all epitopes are known.

    For the most part we do not see cross-species reactivity with sandwich based assays. If you would however nevertheless like to test on of these kits, please do let me know, as I would be able to provide you with an interesting testing offer.

    Please do also let me know whether you would like to discuss other options. Indeed, we have many individual antibodies against leptin in our catalog as well. Some of them are tested in pig (ab3583 and ab16227) and others are tested in ELISA. Again, we could discuss options and provide testing offers if you are interested in this.

    The testing offers would be along the line of the offer described here:
    https://www.abcam.com/collaborationdiscount

    I hope this information is helpful. I am looking forward to hear back from you.

    Read More

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