小鼠KIM1 ELISA试剂盒(TIM-1) (ab119596)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠KIM1 ELISA试剂盒(TIM-1)
参阅全部 TIM 1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 87pg/ml 6.87 = 7.9% 2 16 279pg/ml 14.78 = 5.3% 3 16 842pg/ml 36.2 = 4.3% 批次间 样品 n Mean SD CV% 1 24 85pg/ml 7.31 = 8.6% 2 24 261pg/ml 14.87 = 5.7% 3 24 792pg/ml 38.8 = 4.9% -
样品类型
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
31.2 pg/ml - 2000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s mouse KIM-1 (TIM-1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse KIM-1 (TIM-1) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
A KIM-1 (TIM-1) specific goat polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and are incubated. A biotinylated detection polyclonal antibody from goat specific for KIM-1 (TIM-1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse KIM-1 (TIM-1) amount of sample captured in plate.
Get higher sensitivity in only 90 minutes with Mouse KIM-1 ELISA Kit (TIM1) (ab213477) from our SimpleStep ELISA® range.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-mouse KIM1 antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-Mouse KIM1 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Mouse KIM1 standard 2 x 10ng 2 x 10ng Plate Seal 1 x 4 units 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究领域
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功能
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. -
组织特异性
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. -
序列相似性
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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别名
- CD365
- HAVCR
- HAVCR 1
see all -
数据库链接
- Entrez Gene: 171283 Mouse
- SwissProt: Q5QNS5 Mouse
相关产品
-
SimpleStep ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119596于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve for ab119596
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab119596 被引用在 12 文献中.
- Wu W et al. Treatment with quercetin inhibits SARS-CoV-2 N protein-induced acute kidney injury by blocking Smad3-dependent G1 cell-cycle arrest. Mol Ther 31:344-361 (2023). PubMed: 36514292
- Alherz FA et al. Protective Role of Betulinic Acid against Cisplatin-Induced Nephrotoxicity and Its Antibacterial Potential toward Uropathogenic Bacteria. Pharmaceuticals (Basel) 16:N/A (2023). PubMed: 37631096
- Nguyen LT et al. Blood DNA Methylation Predicts Diabetic Kidney Disease Progression in High Fat Diet-Fed Mice. Nutrients 14:N/A (2022). PubMed: 35215435
- Al-Bataineh MM et al. KIM-1-mediated anti-inflammatory activity is preserved by MUC1 induction in the proximal tubule during ischemia-reperfusion injury. Am J Physiol Renal Physiol 321:F135-F148 (2021). PubMed: 34151589
- Jiménez-Castilla L et al. Nephroprotective Effects of Synthetic Flavonoid Hidrosmin in Experimental Diabetic Nephropathy. Antioxidants (Basel) 10:N/A (2021). PubMed: 34943023