小鼠IP-10 ELISA试剂盒，Fluorescent (ab272186)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 50.32 pg/ml
- Range: 64.22 pg/ml - 32880 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IP-10 ELISA试剂盒，Fluorescent
参阅全部 IP10 试剂盒 -
检测方法
Fluorescent -
精确度
批次内 样品 n Mean SD CV% Supernatant 8 8% 批次间 样品 n Mean SD CV% Supernatant 3 10.6% -
样品类型
Cell culture supernatant, Serum, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
50.32 pg/ml -
范围
64.22 pg/ml - 32880 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 104 93% - 115% Serum 95 92% - 102% Cit plasma 89 84% - 99% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse -
产品概述
IP-10 in vitro CatchPoint SimpleStep ELISA kit is designed for the quantitative measurement of IP-10 (CXCL10) protein in mouse serum, plasma citrate, and cell culture supernatant samples.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
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说明
C-X-C motif chemokine 10 (CXCL10 or IP-10) is a small 10.8kD protein that is secreted by several cell types in response to interferon-gamma (IFNg). These cell types include monocytes, endothelial cells and fibroblasts. Upon secretion, CXCL10 is cleaved into an 8.7kD biologically active protein to function in chemotaxis for T-cells, NK cells, monocytes/macrophages and dendritic cells. In addition, CXCL10 has antitumor activity through the inhibition of bone marrow colony formation and angiogenesis. CXCL10 elicits its effects by binding to the cell surface chemokine receptor 3 (CXCR3).
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Mouse IP-10 (CXCL10) Capture Antibody 1 x 600µl 10X Mouse IP-10 (CXCL10) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Antibody Diluent 5BR 1 x 6ml Mouse IP-10 (CXCL10) Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
研究领域
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功能
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
细胞定位
Secreted. - Information by UniProt
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别名
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
see all -
数据库链接
- Entrez Gene: 15945 Mouse
- SwissProt: P17515 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272186于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Catchpoint ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse IP-10 standard curve in Sample Diluent NS.The IP-10 standard curve was prepared as described in Section 10. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
数据表及文件
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SDS download
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Datasheet download
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