小鼠Insulin like Growth Factor 1 ELISA试剂盒(IGF1) (ab108874)
Key features and details
- Sensitivity: 0.062 ng/ml
- Range: 0.3 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Insulin like Growth Factor 1 ELISA试剂盒(IGF1)
参阅全部 IGF1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 3.2% 批次间 样品 n Mean SD CV% Overall 9.8% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.062 ng/ml -
范围
0.3 ng/ml - 5 ng/ml -
回收率
96 %
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检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s Insulin like Growth Factor 1 (IGF1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin like Growth Factor 1 levels in plasma, serum and cell culture supernatants.
An IGF1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IGF1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IGF1 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Mouse Insulin like Growth Factor 1 Antibody 1 vial Chromogen Substrate 1 x 7ml Insulin like Growth Factor 1 Microplate (12 x 8 well strips) 1 unit Insulin like Growth Factor 1 Standard 1 vial Sealing Tapes 1 x 3 units Stop Solution 1 x 11ml -
研究领域
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功能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
疾病相关
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
序列相似性
Belongs to the insulin family. -
细胞定位
Secreted. - Information by UniProt
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别名
- IBP1
- IGF I
- IGF IA
see all -
数据库链接
- Entrez Gene: 16000 Mouse
- SwissProt: P05017 Mouse
- Unigene: 268521 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108874于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108874
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108874 被引用在 5 文献中.
- Cai F et al. Transcription factor GTF2B regulates AIP protein expression in growth hormone-secreting pituitary adenomas and influences tumor phenotypes. Neuro Oncol 24:925-935 (2022). PubMed: 34932801
- Rajala A et al. Insulin-like growth factor 1 receptor mediates photoreceptor neuroprotection. Cell Death Dis 13:613 (2022). PubMed: 35840554
- Wu H et al. Whole Body Vibration Retards Progression of Atherosclerosis via Insulin-Like Growth Factor 1 in Apolipoprotein E-Deficient Mice. Biomed Res Int 2018:4934861 (2018). PubMed: 29707570
- Li C et al. Suppression of Basic Fibroblast Growth Factor Expression by Antisense Oligonucleotides Inhibits Neural Stem Cell Proliferation and Differentiation in Rat models With Focal Cerebral Infarction. J Cell Biochem 118:3875-3882 (2017). PubMed: 28390174
- Wang T et al. GDF15 is a heart-derived hormone that regulates body growth. EMBO Mol Med 9:1150-1164 (2017). PubMed: 28572090