JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB227902

小鼠IL-7 ELISA试剂盒

Mouse IL-7 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse IL-7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-7 with a sensitivity of 12.046 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Il-7, Il7, Interleukin-7, IL-7

3 Images
Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)

Mouse IL-7 Standard Curve Comparison Data.

Standard curve comparison between mouse IL-7 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)

Interpolated concentrations of spiked IL-7 in Mouse serum, plasma and cell culture supernatant samples.

The concentrations of IL-7 were measured in duplicates, interpolated from the IL-7 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 50% and splenocyte supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-7 concentration was determined to be 2441.13 pg/mL in serum, 1535.45 pg/mL in plasma (heparin), and 2581.66g/mL in and splenocyte supernatant.

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)

Example of Mouse IL-7 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Cell culture supernatant, Serum

反应种属

Mouse

检测类型

Sandwich

Quantitative
灵敏度

= 12.046 pg/mL

范围

39.06 - 2500 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style
left-column

搭配使用产品

Assay Kits

AB218266

Human IL-15 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-15 ELISA Kit (AB218266)

  • 0
  • 0
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
Assay Kits

AB223592

Human IL-12 p70 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-12 p70 ELISA Kit (AB223592)

  • 0
  • 0
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Mouse IL-7 ELISA Kit (ab227902) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-7 protein in cell culture supernatant, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-7 with 12.046 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Unsuitable for EDTA plasma.

style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Spiked Media", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Spiked Media", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]
style
left-column

回收率

[ { "sample": "Cell culture supernatant", "range": "103 - 105 %", "average": "= 107" }, { "sample": "Serum", "range": "83 - 88 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "81 - 83 %", "average": "= 83" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab227902-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB227902-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB227902-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB227902-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB227902-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB227902-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB227902-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB227902-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-7 Lyophilized Recombinant Protein", "number":"AB227902-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-7 Capture Antibody", "number":"AB227902-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-7 Detector Antibody", "number":"AB227902-CMP07", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-7 (IL-7) also known as IL-7 protein or IL-7 is a cytokine with a critical role in immune system development and function. IL-7 has an approximate molecular mass of 25 kDa and it exhibits expression in several tissues including stromal cells in the bone marrow and thymus. It interacts with its specific receptor IL-7R which consists of the IL-7Rα chain and the common gamma chain (γc) shared with other cytokine receptors. These interactions are important for signaling pathways that support lymphoid tissue homeostasis.
Biological function summary

IL-7 significantly influences lymphocyte development and survival. It is essential for the proliferation and maintenance of T cells particularly thymocytes during T cell maturation and peripheral T cells after they exit the thymus. IL-7 does not form a complex itself but works as a critical single molecule in these processes. IL-7's role is especially noted in the maintenance of naïve and memory T cells important for adaptive immunity.

Pathways

IL-7 activity impacts important immune signaling routes. The cytokine is an important player in the JAK/STAT signaling pathway which gets activated upon IL-7R engagement. This pathway is fundamental in controlling T cell development proliferation and survival. IL-7's interaction with other proteins such as the IL-7R complex and their downstream signaling partners integrates it into networks critical for strengthening the immune response.

IL-7 is significantly linked to immunodeficiencies and cancers affecting immune cells. For instance IL-7's altered signaling is associated with severe combined immunodeficiencies (SCID) where the lack of functional IL-7 or its receptor compromises T cell development. Additionally overexpression of IL-7 has connections to certain leukemias and lymphomas where it may contribute to inappropriate lymphocyte proliferation. In these contexts IL-7 often gets investigated alongside proteins such as the JAK and STAT family involved in tumorigenesis and immune dysregulation.
style
left-column

产品实验方案

style
left-column

靶点信息

Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis (PubMed : 28811625, PubMed : 7699333). Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.
See full target information Il7
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com