AB100714

小鼠IL-7 ELISA试剂盒

Name: 小鼠IL-7 ELISA试剂盒 (ab100714)| Abcam中文官网
Brand: Abcam Limited
SKU: AB100714
Price: 7672 CNY
Availability: InStock

Mouse IL-7 ELISA Kit

Be the first to review this product! Submit a review

(2 Publications)

Mouse IL-7 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-7 in Mouse in Biofluids, Cell Culture Media samples.

查看别名

Il-7, Il7, Interleukin-7, IL-7

2 Images

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB100714)

sELISA

Unknown

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB100714)

Representative standard curve using ab100714

sELISA

Unknown

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB100714)

Representative standard curve using ab100714

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Mouse

检测类型

Sandwich (quantitative)

灵敏度

< 10 pg/mL

范围

8.23 - 6000 pg/mL

检测平台

Microplate

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

产品详情

Abcam's IL-7 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-7 in serum, plasma and cell culture supernates.

This assay employs an antibody specific for mouse IL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style

left-column

回收率

[ { "sample": "Cell culture supernatant", "range": "85 - 105 %", "average": "= 94.58" }, { "sample": "Serum", "range": "82 - 103 %", "average": "= 91.59" }, { "sample": "Plasma", "range": "83 - 104 %", "average": "= 92.66" } ]

style

left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100714-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100714-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100714-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-7 Microplate (12 x 8 wells)", "number":"AB100714-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100714-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant mouse IL-7 Standard (lyophilized)", "number":"AB100714-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100714-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"440X HRP-Streptavidin Concentrate", "number":"AB100714-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse IL-7", "number":"AB100714-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100714-CMP06", "productcode":"" } ] } } }

style

left-column

性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-7 (IL-7) also known as IL-7 protein or IL-7 is a cytokine with a critical role in immune system development and function. IL-7 has an approximate molecular mass of 25 kDa and it exhibits expression in several tissues including stromal cells in the bone marrow and thymus. It interacts with its specific receptor IL-7R which consists of the IL-7Rα chain and the common gamma chain (γc) shared with other cytokine receptors. These interactions are important for signaling pathways that support lymphoid tissue homeostasis.

Biological function summary

IL-7 significantly influences lymphocyte development and survival. It is essential for the proliferation and maintenance of T cells particularly thymocytes during T cell maturation and peripheral T cells after they exit the thymus. IL-7 does not form a complex itself but works as a critical single molecule in these processes. IL-7's role is especially noted in the maintenance of naïve and memory T cells important for adaptive immunity.

Pathways

IL-7 activity impacts important immune signaling routes. The cytokine is an important player in the JAK/STAT signaling pathway which gets activated upon IL-7R engagement. This pathway is fundamental in controlling T cell development proliferation and survival. IL-7's interaction with other proteins such as the IL-7R complex and their downstream signaling partners integrates it into networks critical for strengthening the immune response.

IL-7 is significantly linked to immunodeficiencies and cancers affecting immune cells. For instance IL-7's altered signaling is associated with severe combined immunodeficiencies (SCID) where the lack of functional IL-7 or its receptor compromises T cell development. Additionally overexpression of IL-7 has connections to certain leukemias and lymphomas where it may contribute to inappropriate lymphocyte proliferation. In these contexts IL-7 often gets investigated alongside proteins such as the JAK and STAT family involved in tumorigenesis and immune dysregulation.

style

left-column

产品实验方案

style

left-column

靶点信息

Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis (PubMed : 28811625, PubMed : 7699333). Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.

See full target information Il7

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Canadian journal of gastroenterology & hepatology 2019:9897315 PubMed30941331

2019

Copper Mediates Anti-Inflammatory and Antifibrotic Activity of Gleevec in Hepatocellular Carcinoma-Induced Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Iftekhar Hassan,Hossam Ebaid,Ibrahim M Alhazza,Jameel Al-Tamimi,Shazia Aman,Ahmad M Abdel-Mageed

Infection and immunity 84:108-19 PubMed26483405

2015

Treatment with Interleukin-7 Restores Host Defense against Pneumocystis in CD4+ T-Lymphocyte-Depleted Mice.

Applications

Unspecified application

Species

Unspecified reactive species

S Ruan,D R Samuelson,B Assouline,M Morre,J E Shellito

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

小鼠IL-7 ELISA试剂盒

Mouse IL-7 ELISA Kit

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB100714)

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB100714)