AB100711

小鼠IL-5 ELISA试剂盒

Name: 小鼠IL-5 ELISA试剂盒 (ab100711)| Abcam中文官网
Brand: Abcam Limited
SKU: AB100711
Price: 7672 CNY
Availability: InStock

Mouse IL-5 ELISA Kit

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(3 Publications)

Mouse IL-5 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-5 in Mouse in Biofluids, Cell Culture Media samples.

查看别名

Il-5, Il5, Interleukin-5, IL-5, B-cell growth factor II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, BCGF-II, TRF

2 Images

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB100711)

sELISA

Unknown

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB100711)

Representative standard curve using ab100711

sELISA

Unknown

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB100711)

Representative standard curve using ab100711

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Mouse

检测类型

Sandwich

灵敏度

< 1 pg/mL

范围

2.05 - 500 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's IL-5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-5 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get better reproducibility in only 90 minutes with Mouse IL-5 ELISA Kit (ab204523) from our SimpleStep ELISA^® range.

The standard is affinity purified.

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回收率

[ { "sample": "Cell culture supernatant", "range": "85 - 104 %", "average": "= 95.76" }, { "sample": "Serum", "range": "82 - 102 %", "average": "= 93.44" }, { "sample": "Plasma", "range": "93 - 104 %", "average": "= 95.49" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100711-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100711-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100711-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100711-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse IL-5", "number":"AB100711-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100711-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100711-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant mouse IL-5 Standard (lyophilized)", "number":"AB100711-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100711-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-5 Microplate (12 x 8 wells)", "number":"AB100711-CMP03", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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产品实验方案

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靶点信息

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 10444455, PubMed : 1873482). Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (PubMed : 3128631). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (By similarity).

See full target information Il5

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 18:88 PubMed36184652

2022

Anti-asthmatic miR-224-5p inhibits the FHL1/MAPK pathway to repress airway smooth muscle cell proliferation in a murine model of asthma-like airway inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifang Zhuang,Yanjuan Zhou,Jiao Xu,Leying Pan

Clinical, cosmetic and investigational dermatology 14:1675-1683 PubMed34815684

2021

Gallic Acid Ameliorates Atopic Dermatitis-Like Skin Inflammation Through Immune Regulation in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Guohong Hu,Xiansheng Zhou

American journal of translational research 11:280-289 PubMed30787986

2019

Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Kong,Jia Yin

View all publications

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websiteProtocolBooklet

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Abcam Product Promise

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小鼠IL-5 ELISA试剂盒

Mouse IL-5 ELISA Kit

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB100711)

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB100711)