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AB208989

小鼠IL-28A ELISA试剂盒

Mouse IL-28A ELISA Kit

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Mouse IL-28A ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-28A with a sensitivity of 2.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Il28a, Ifnl2, Interferon lambda-2, IFN-lambda-2, Interleukin-28A, IL-28A

3 Images
Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)

Interpolated concentrations of spiked IL-28A in mouse serum and plasma.

The concentrations of IL-28A were measured in duplicates, interpolated from the (target) standard curves and corrected for sample dilution. 1X diluted samples are as follows : serum 12.5% and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)

Interpolated concentrations of spiked IL-28A in cell culture supernatant samples.

The concentrations of IL-28A were measured in duplicates, interpolated from the (target) standard curves and corrected for sample dilution. 1X diluted samples are as follows : lung cell culture supernatant 25%, and RAW 264.7 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)

Example of mouse IL-28A standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Mouse

检测类型

Sandwich

Quantitative
灵敏度

= 2.4 pg/mL

范围

15.63 - 1000 pg/mL

检测时间

1h 30m

检测平台

Microplate (12 x 8 well strips)

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搭配使用产品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB245076

Anti-IL-28A antibody [EPR20974-48] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-28A antibody [EPR20974-48] - BSA and Azide free (Detector) (AB245076)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Assay Kits

AB100708

Mouse IL-28 ELISA Kit

Sandwich ELISA - Mouse IL-28 ELISA Kit (AB100708)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Mouse IL-28A ELISA Kit (ab208989) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-28A protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-28A with 2.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-28A (Interleukin 28A), also known as IFN-lambda 2 (Interferon-lambda 2), is a class II cytokine receptor ligand with antiviral, antitumor, and immunomodulatory activities.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "102 - 107 %", "average": "= 105" }, { "sample": "Serum", "range": "110 - 112 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "106 - 106 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "109 - 112 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "106 - 108 %", "average": "= 108" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab208989-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB208989-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB208989-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB208989-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB208989-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB208989-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB208989-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB208989-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-28A Lyophilized Recombinant Protein", "number":"AB208989-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-28A Capture Antibody", "number":"AB208989-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-28A Detector Antibody", "number":"AB208989-CMP02", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information Ifnl2
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websiteProtocolBooklet
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