小鼠IL-22 ELISA试剂盒
Mouse IL-22 ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
Be the first to review this product! Submit a review
|
(4 Publications)
Mouse IL-22 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-22 with a sensitivity of 0.15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
Il22a, Iltif, Iltifa, Il22, Interleukin-22, IL-22, IL-10-related T-cell-derived-inducible factor, IL-TIF alpha, Interleukin-22a, IL-TIF, IL-22a
- ELISA
Supplier Data
ELISA - Mouse IL-22 ELISA Kit (AB223857)
- sELISA
Supplier Data
Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)
Interpolated concentrations of native IL-22 in stimulated EL4.IL2 and spleen cell culture supernatant.
The concentrations of IL-22 were measured in duplicate and interpolated from the IL-22 standard curve and corrected for sample dilution. Undiluted samples are as follows : EL4.IL2 supernatant 25% and spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 500 pg/mL in EL4.IL2 supernatant and 225 pg/mL in spleen supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)
Interpolated concentrations of native IL-22 in Mouse serum and plasma samples.
The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 117 pg/mL in serum, 101 pg/mL in plasma (citrate), 193 pg/mL in plasma (heparin), and 137 pg/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)
Comparison of IL-22 in unstimulated and stimulated cell culture supernatants.
EL4.IL2 cells were cultured to semi-confluency and then treated for 48 hours with either 10 μg/mL PHA + 10 ng/mL PMA or DMSO (mock). Rat spleen cells were cultured in the presence or absence of 100 ng/mL IFN-gamma + 1 μg/mL LPS for 4 days. The concentrations of IL-22 were measured in five different dilutions of the supernatant samples in duplicates and interpolated from the IL-22 standard curve. The interpolated values are plotted (mean +/- SD, n=5) in pg per mL of neat supernatant. The mean IL-22 concentration was determined to be 500 pg/mL in stimulated EL4.IL2 supernatant and undetectable in unstimulated EL4.IL2 supernatant. The mean concentration was determined to be 225 pg/mL in stimulated spleen supernatant and 1.7 pg/mL in unstimulated spleen supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)
Example of Mouse IL-22 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Mouse IL-22 ELISA Kit (AB223857)
Mouse IL-22 standard curve comparison data.
Standard curve comparison between mouse IL-22 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
搭配使用产品
反应性数据
产品详情
Mouse IL-22 ELISA Kit ab223857 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-22 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.15 pg/mL.
How the assay works
Mouse IL-22 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse IL-22 ELISA Kit ab223857 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IL-22 antibody [EPR20468-273] - BSA and Azide free (Capture) ab244928
- Anti-IL-22 antibody [EPR20468-222] - BSA and Azide free (Detector) ab244928
Interleukin 22 (IL-22) is a cytokine thought to be involved in the response to inflammation through the Jak/STAT and MAPK signaling pathways. IL-22 is produced by NK cells and Th1-type T cells and can stimulate the induction of pro-inflammatory cytokines in human keratinocytes.
精确度
回收率
规格
性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
靶点信息
文献 (4)
Recent publications for all applications. Explore the full list and refine your search
Science advances 11:eadt6113 PubMed40249812
2025
Applications
Unspecified application
Species
Unspecified reactive species
Chinese medicine 19:81 PubMed38858762
2024
Applications
Unspecified application
Species
Unspecified reactive species
Asian Pacific journal of cancer prevention : APJCP 22:3647-3661 PubMed34837924
2021
Applications
Unspecified application
Species
Unspecified reactive species
RSC advances 10:3371-3379 PubMed35497743
2020
Applications
Unspecified application
Species
Unspecified reactive species
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com