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AB282870

小鼠IL-21 ELISA试剂盒

Mouse IL-21 ELISA Kit

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(1 Publication)

Mouse IL-21 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-21 with a sensitivity of 10.45 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Interleukin-21, IL-21, Il21

2 Images
Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)

Interpolated concentrations of spiked IL-21 in mouse serum and plasma EDTA.

The concentrations of IL-21 were measured in duplicates, interpolated from the IL-21 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)

Example of mouse IL-21 standard curve in Sample Diluent 75BP.

The IL-21 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, EDTA Plasma

反应种属

Mouse

检测类型

Sandwich

Quantitative
灵敏度

= 10.45 pg/mL

范围

62.5 - 4000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Mouse IL-21 ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse IL-21 protein in mouse serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-21 with 10.45 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse IL-21 is a cytokine involved in the regulation between the innate and adaptive immunity response. It is primarily expressed by activated T-cells. It has been suggested to have anti-tumor activity and may be involved in the development of autoimmunity.

精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]

回收率

[ { "sample": "Serum", "range": "95 - 105 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "113 - 120 %", "average": "= 116" } ]

规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C

产品实验方案

靶点信息

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (PubMed : 15100251). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15 (PubMed : 11970879). May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (PubMed : 12893770).
See full target information Il21

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 20:83 PubMed35658901

2022

Lactobacillus improves the effects of prednisone on autoimmune hepatitis via gut microbiota-mediated follicular helper T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Ma,Liwen Zhang,Yun Zhuang,Yanbo Ding,Jianping Chen
View all publications
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