AB267643

小鼠IL-2 R alpha ELISA试剂盒

Name: 小鼠IL-2 R alpha ELISA试剂盒 (ab267643)| Abcam中文官网
Brand: Abcam Limited
SKU: AB267643
Price: 8467 CNY
Availability: InStock

Mouse IL-2 R alpha ELISA Kit

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Mouse IL-2 R alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-2 R alpha in Mouse in Biofluids, Cell Culture Media samples.

查看别名

CD25, Il2r, Il2ra, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55

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Sandwich ELISA - Mouse IL-2 R alpha ELISA Kit (AB267643)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-2 R alpha ELISA Kit (AB267643)

This standard curve is for demonstration only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Mouse

检测类型

Sandwich (quantitative)

灵敏度

= 10 pg/mL

范围

10.24 - 2500 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Mouse IL-2 R alpha ELISA Kit is designed for the quantitative determination of IL-2 R alpha in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for mouse IL-2 R alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 R alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-2 R alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 R alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit detects mouse IL-2R alpha, less than 0.1% cross-reactivity with human IL-2R alpha is observed.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]

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回收率

[ { "sample": "Serum", "range": "85 - 105 %", "average": "= 96.14" }, { "sample": "Plasma", "range": "68 - 107 %", "average": "= 82.55" }, { "sample": "Cell culture media", "range": "92 - 124 %", "average": "= 105.7" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267643-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267643-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-2 R alpha Standard (Lyophilized)", "number":"AB267643-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267643-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse IL-2 R alpha coated Microplate (12 x 8 wells)", "number":"AB267643-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267643-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB267643-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267643-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse IL-2 R alpha Detection Antibody", "number":"AB267643-CMP08", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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产品实验方案

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靶点信息

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

See full target information Il2ra

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websiteProtocolBooklet

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小鼠IL-2 R alpha ELISA试剂盒

Mouse IL-2 R alpha ELISA Kit

Sandwich ELISA - Mouse IL-2 R alpha ELISA Kit (AB267643)